The chemical differences between equine and bovine heart-derived Cytochrome C for cytochrome oxidase histochemistry are minimal. The molecular weights are close but not identical, with equine heart-derived Cytochrome C having a molecular weight of 12,384 and bovine heart-derived Cytochrome C having a molecular weight of 12,327. Despite this, both are highly conserved in eukaryotes, and considering their near-identical molecular weights, they can likely be used interchangeably for cytochrome oxidase histochemistry.
C2506
シトクロムC from equine heart
≥95% (SDS-PAGE)
別名:
シトクロムC ウマ心臓由来
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About This Item
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由来生物
horse heart
品質水準
アッセイ
≥95% (SDS-PAGE)
フォーム
powder
分子量
12,384
テクニック
cell based assay: suitable
溶解性
H2O: soluble 10 mg/mL
適合性
suitable for molecular biology
UniProtアクセッション番号
アプリケーション
cell analysis
保管温度
−20°C
遺伝子情報
horse ... CYCS(100053958)
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アプリケーション
生物化学的/生理学的作用
調製ノート
その他情報
アプリケーション
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C2506-500MG:
C2506-100MG:
E002506-50MG:
C2506-2G:
C2506-VAR:
C2506-1G:
E002506-BULK:
C2506-5G:
C2506-250MG:
C2506-50MG:
E002506-250MG:
C2506-BULK:
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試験成績書(COA)
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Chromatograms
application for HPLCapplication for HPLCapplication for HPLC-
What are the chemical differences between equine and bovine heart-derived Cytochrome C for cytochrome oxidase histochemistry?
1 回答-
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Does the solubilized powder have a particular color (compared to bovine cytC, which has a brown color)?
1 回答-
This product in solution should be dark red to reddish brown when dissolved in water at 10 mg/mL. See below for a link to the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C2506-BULKSLCL7912.pdf役に立ちましたか?
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか?
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How do I determine how much of Product C2506, Cytochrome c, is in the oxidized form, and how much is reduced?
1 回答-
This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service ([email protected]).
役に立ちましたか?
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Is Product C2506, Cytochrome c, predominantly in the oxidized form or the reduced form?
1 回答-
This product is predominantly (>95%) in the oxidized form.
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How should I store solutions of Product C2506, Cytochrome c?
1 回答-
Our general recommendations are as follows: Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.
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I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?
1 回答-
Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required. Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system. Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.
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ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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