Product No. C2506 is prepared with trichloroacetic acid (TCA), whereas C7752 is prepared with the milder acetic acid. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Product No. C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. It is recommended when an ultra-high purity is required. Product No. C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. This fraction shows a high rate of ascorbic acid oxidation, but low activity in a cytochrome oxidase system. Product No. C7150 is also prepared from C7752; it is suitable for use as a gel filtration molecular weight marker.
C7752
シトクロムC from equine heart
≥95% based on Mol. Wt. 12,384 basis
別名:
シトクロムC ウマ心臓由来
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
50 mg | 在庫from市川市大町368 | ￥38,800 |
100 mg | 在庫from市川市大町368 | ￥59,600 |
500 mg | 在庫from市川市大町368 | ￥214,000 |
1 g | 在庫from市川市大町368 | ￥355,000 |
5 g | カートの在庫状況を確認する | ￥912,000 |
この商品について
biological source
horse heart
Quality Segment
assay
≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis
form
powder
mol wt
12,384
technique(s)
cell culture | mammalian: suitable
suitability
suitable for molecular biology
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
Application
チートクロムcは、ブルーネイティブゲル電気泳動（BN-PAGE）を用いて複合体IV活性を検出および評価するためのリン酸緩衝液の成分としての使用に適しています。また、単離された腎食細胞で細胞外スーパーオキシドアニオン（ECSA）を検出するためにも使用できます。
Biochem/physiol Actions
Preparation Note
Other Notes
1 of 1
当該品目 | |||
|---|---|---|---|
| assay ≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis | assay ≥99% (SDS-PAGE) | assay - | assay ≥95% (SDS-PAGE) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable, mass spectrometry (MS): suitable, western blot (chemiluminescent): suitable |
| biological source horse heart | biological source horse heart | biological source - | biological source human heart |
| mol wt 12,384 | mol wt 12,384 | mol wt ~12,400 | mol wt 11 kDa by SDS-PAGE |
| form powder | form powder | form powder | form lyophilized powder |
| suitability suitable for molecular biology | suitability - | suitability - | suitability - |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
C7752-50MG: + C7752-BULK: + C7752-5G: + C7752-500MG: + C7752-25MG: + C7752-100MG: + C7752-VAR: + C7752-250MG: + C7752-1G: + C7752-RSAMPLE: + C7752-EW:
jan
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I see that you list several horse heart cytochrome c products. What are the differences, and which one should I choose?
1 回答-
役に立ちましたか？
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How do I determine how much of Product C7752, Cytochrome c, is in the oxidized form, and how much is reduced?
1 回答-
This is determined by a spectral assay. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service ([email protected]).
役に立ちましたか？
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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How should I store solutions of Product C7752, Cytochrome c?
1 回答-
Our general recommendations are as follows: Store at -20 °C (freezer) for up to six months. Store at 2-8 °C (refrigerator) for up to two weeks. Store at 20-25 °C (room temperature) for up to three days. According to J. Biol. Chem, 237, 2161-2174 (1962), cytochrome c is stable to boiling, and over an unusually wide range of pH values. However, it denatures readily at extreme pH (low or high), and in high concentrations of organic solvents, such as ethanol or acetone.
役に立ちましたか？
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Is Product C7752, Cytochrome c, predominantly in the oxidized form or the reduced form?
1 回答-
This product is predominantly (>95%) in the oxidized form.
役に立ちましたか？
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