C0678

モノクロナール抗HNK-1/N-CAM (CD57) マウス宿主抗体

clone VC1.1, ascites fluid, buffered aqueous solution

• 別名: モノクロナール抗HNK-1, 抗CD57抗体, 抗N-CAM抗体
• MDL番号: MFCD00164634
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: VC1.1, monoclonal
• フォーム: buffered aqueous solution
• 含みます: 15 mM sodium azide
• 交差性: mouse, rat, feline, human
• テクニック: western blot: 1:4,000 using cerebral cortex extract
• アイソタイプ: IgM
• UniProtアクセッション番号: Q9P2W7
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... B3GAT1(27087) ; mouse ... B3gat1(76898) ; rat ... B3gat1(117108)

詳細

詳細

Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.

特異性

ヒト骨髄細胞に関連する細胞表面糖タンパク質CD57/HNK-1を認識します。認識されるエピト-プは、多くの糖タンパク質やある種の糖脂質に存在するN-結合型糖鎖です。ホルマリン固定およびパラフィン包埋に安定です。VC1.1抗体とHNK-1(Leu7)抗体は、それぞれの結合を阻害します。本抗体は、ある種のミエリン結合糖タンパク質や高分子量コンドロイチン硫酸プロテオグリカンを認識します。
5th Workshop: code no. NK 67

免疫原

ネコ一次視覚野(領域17)のホモジネ-ト。

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:

• as primary antibody for western blot analysis
• immunocytochemistry
• immunocytochemical
• immunohistological staining

生物化学的／生理学的作用

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

ターゲットの説明

CD57/HNK-1は末梢血単核細胞亜集団の15-20%、NK活性化細胞の約60%をはじめ、T細胞サブセットに発現しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable