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由来生物
avidin from egg white
enzyme from bovine (calf) intestine
結合体
alkaline phosphatase conjugate
フォーム
buffered aqueous solution
テクニック
direct ELISA: 1:70,000
western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane
輸送温度
wet ice
保管温度
2-8°C
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詳細
Avidin is homotetrameric protein (66 kDa) obtained from egg whites and binds strongly to biotin. It has four identical subunits of 16,400 Daltons each.[1] It is an attractive adaptor protein, that is resistant to denaturation.[2] This glycoprotein is present in avian, reptilian and amphibian egg white.[3] The product is affinity purified egg white avidin (acitivity 10-15 units/mg protein) conjugated to alkaline phosphatase using a 0.2% glutaraldehyde method.
Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[4]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
アプリケーション
Avidin-Alkaline Phosphatase has been used for ELISA[5][6]. The product can also be used for western blot at 1:150,000-1:300,000 dilutions.
Cytokine ELISA Assays were performed using a biotinylated anti-IL-2 antibody and alkaline phosphatase avidin. [9]
生物化学的／生理学的作用
Avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities. Avidin is a fatty acid biosynthesis regulator. It participates in terminal cell differentiation by weakening the multiplication of cell without affecting the differentiation process.[3]
物理的形状
0.05M Trisバッファ-溶液 (pH 8.0, 1%BSA, 1mM MgCl2, 15mMアジ化ナトリウム含有)。
調製ノート
0.2%グルタルアルデヒドを用いてアルカリホスファタ-ゼとコンジュゲ-ションした精製タンパク質です。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A7294-1ML:
A7294-BULK:
A7294-VAR:
最新バージョンのいずれかを選択してください：
試験成績書（COA）
Lot/Batch Number
Ana Maria Marassá et al.
Revista da Sociedade Brasileira de Medicina Tropical, 39(2), 183-186 (2006-05-16)
Lutzomyia longipalpis and Lutzomyia almerioi, phlebotomine species from the fauna of Serra da Bodoquena, in the State of Mato Grosso do Sul, Brazil, have been studied, particularly due to the fact of their abundance and occurrence, the Guaicurus settlement, focus
Avidin expression during chick chondrocyte and myoblast development in vitro and in vivo: regulation of cell proliferation
Zerega B, et al.
Journal of Cell Science, 114(8), 1473-1482 (2001)
(Strept)avidin-Biotin Systems
Bioconjugate Techniques, 465-505 (2013)
Françoise Immel et al.
PloS one, 11(5), e0154264-e0154264 (2016-05-24)
The zebra mussel Dreissena polymorpha is a well-established invasive model organism. Although extensively used in environmental sciences, virtually nothing is known of the molecular process of its shell calcification. By describing the microstructure, geochemistry and biochemistry/proteomics of the shell, the
Kaitlyn Grando et al.
Frontiers in cellular and infection microbiology, 12, 884065-884065 (2022-06-02)
The bacterial amyloid curli, produced by Enterobacteriales including Salmonella species and Escherichia coli, is implicated in the pathogenesis of several complex autoimmune diseases. Curli binds to extracellular DNA, and these complexes drive autoimmunity via production of anti-double-stranded DNA autoantibodies. Here
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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