A4213

モノクローナル抗マルトース結合タンパク質抗体-ペルオキシダーゼ標識 マウス宿主抗体

clone MBP-17, purified immunoglobulin, lyophilized powder

• 別名: モノクロナール抗マルトース結合タンパク質, 抗MBP抗体
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: peroxidase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: MBP-17, monoclonal
• フォーム: lyophilized powder
• 包装: vial of 0.5 mL
• テクニック: western blot: 1:1,000 using purified, recombinant MBP
• アイソタイプ: IgG1
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

抗体は、未変性および変性・還元型の精製MBPやMBP融合タンパク質を認識します。

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the MBP-17 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified recombinant MBP fusion protein. Maltose Binding Protein (MBP) is a periplasmic protein, which is specific to bacteria. It has maltodextrin binding site and binds to linear and cyclic maltodextrins. The mature MBP is produced from the pre-protein containing 26 amino acids, as signal peptide.

免疫原

精製したリコンビナントMBP融合タンパク質。

アプリケーション

Endogenous intracellular levels of MBP in salmonella cells were determined by western blot analysis using a monoclonal anti-MBP antibody.

Monoclonal Anti-Maltose Binding Protein Peroxidase antibody produced in mouse has been used in:

• immunoblotting
• dot blot
• enzyme-linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Maltose Binding Protein (MBP) is often used to tag recombinant proteins to enhance the yield and facilitate the purification of the fusion product. Additionally, MBP tags can also improve the immunogenicity of the tagged protein. Thus, MBP can also be used as a carrier protein for vaccinations.

Maltose Binding Protein (MBP) mediates uptake of maltose. MBP is a substrate for chaperone secB. It has been reported that the addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product.

物理的形状

Lyophilized in 0.01 M sodium phosphate buffered saline containing 1% bovine serum albumin and 0.05% MIT.

調製ノート

Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978)によって報告された2段階グルタルアルデヒド法により作製しています。

再構成

標識抗体は0.5 mLの脱イオン水で復元してください。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable