7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester (7-OHCCA-SE) is used as a reagent to conjugate 7-Hydroxycoumarin-3-carboxylic acid (7-OHCCA) to other molecules such as amino acids via amide chemistry.
Proceedings of the National Academy of Sciences of the United States of America, 95(13), 7396-7401 (1998-06-24)
The chromophore of photoactive yellow protein (PYP) (i.e., 4-hydroxycinnamic acid) has been replaced by an analogue with a triple bond, rather than a double bond (by using 4-hydroxyphenylpropiolic acid in the reconstitution, yielding hybrid I) and by a "locked" chromophore
A fluorometric binding assay for lectin and yeast cells using the avidin-biotin system was previously reported (Y. Oda, M. Kinoshita, and K. Kakehi, Anal. Biochem. 254, 41-48, 1997). However, the true amount of bound lectin could not be determined by