This supplement product is tested in growth promotion assays at 10% in medium using fairly robust internals stock cell lines, such as VERO and BHK. The concentration may need to be optimized for other cell lines.
Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/225/678/p14008.pdf
現在、価格および在庫状況を閲覧できません。
おすすめの製品
無菌性
γ-irradiated by SER-TAIN™ process (FBS)
sterile; sterile-filtered
フォーム
solution
輸送温度
dry ice
保管温度
−20°C
詳細
SERUM PLUS™ contains a low level of gamma irradiated Fetal Bovine Serum (FBS) supplemented with proteins that promote nutrient transport, cell attachement, and cell growth.
アプリケーション
特徴および利点
Benefits include less than half of the total protein concentration found in serum, reduced levels of inhibitors such as proteases and viral antibodies, and consistency of lot-to-lot performance due to greater control of the component materials.
調製ノート
Thawing
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.
Note:
Gentle, periodic swirling of this product is crucial for optimum performance. If a bottle is not periodically swirled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.
Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.
1. Remove the supplement bottles from the freezer and allow them to acclimate to room temperature for approximately 10 minutes.
2. Place each container in a 30 - 37 °C water bath or incubator. Excessive temperatures will degrade heat liable nutrients. If using a water bath, prevent the bottle caps from being completely submerged.
3. Gently swirl the bottles every 10-15 minutes until the serum is completely thawed.
4. After thawing, use the supplement promptly. Liquid supplement may be stored refrigerated (2 - 8 °C) up to four weeks. To avoid freeze/thaw cycles or long periods of refrigeration, it is recommended that any unused supplement be immediately dispensed into small, useful aliquots and refrozen for future use.
Note:
Gentle, periodic swirling of this product is crucial for optimum performance. If a bottle is not periodically swirled as it thaws, gradients containing high concentrations of salts, proteins and lipids will form throughout the liquid portion and lead to the formation of crystalline or flocculent precipitates. These cryoprecipitates are not toxic to cell cultures, but they affect the appearance and consistency of each bottle.
Small amounts of cryoprecipitates are not uncommon, and will not affect product performance. Gently warming and mixing the supplement will generally allow the material to go back into solution.
法的情報
SER-TAIN is a trademark of Sigma-Aldrich Co. LLC
SERUM PLUS is a trademark of Sigma-Aldrich Co. LLC
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
14008C-500ML:
14008CPROC:
14008C-100ML:
14008-500M:
14008-500M3284:
14008-100M:
14008C-BULK:
14008C-PH:
この製品を見ている人はこちらもチェック
Utilizing Chemical Genomics to Identify Cytochrome b as a Novel Drug Target for Chagas Disease.
Khare S et al.
PLoS Pathogens, 11, e1005058-e1005058 (2015)
Proteasome inhibition for treatment of leishmaniasis, Chagas disease and sleeping sickness.
Khare S et al.
Nature, 537, 229-229 (2016)
Joseph Verdi et al.
Cell host & microbe, 28(1), 79-88 (2020-05-18)
Trypanosomiasis is a devastating neglected tropical disease affecting livestock and humans. Humans are susceptible to two Trypanosoma brucei subspecies but protected from other trypanosomes by circulating high-density lipoprotein (HDL) complexes called trypanosome lytic factors (TLFs) 1 and 2. TLFs contain
Nidhi Gupta et al.
Journal of the American Society of Nephrology : JASN, 31(9), 2065-2082 (2020-08-09)
Circulating APOL1 lyses trypanosomes, protecting against human sleeping sickness. Two common African gene variants of APOL1, G1 and G2, protect against infection by species of trypanosomes that resist wild-type APOL1. At the same time, the protection predisposes humans to CKD
資料
Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.
-
How much Serum plus medium supplement (14008C) is added to base medium? Is it equivalent to standard FBS (i.e., 10% of volume)?
1 回答-
役に立ちましたか？
-
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）
