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製品名
Caco-2 Engineered Control Cells, one assay ready, 24-well plate
由来生物
human male colorectal tissue (Source Disease: adenocarcinoma)
フォーム
liquid
詳細
The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).
The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt.
A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process.
The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that is shipped at room temperature the cells must be processed immediately upon receipt.
A minimum of 4 assay ready plates is required to purchase. Please allow for 1 week after ordering, for the plates to be seeded. Plates will be shipped to the customer on day 16 of the 21-day culturing process.
アプリケーション
The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases
Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes
Caco-2 Transporter Knockout Cell Based Assays
特徴および利点
The Caco-2 subclone, C2BBe1 Engineered Control cells, are ideal for permeability and transporter analyses as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis. Other benefits include:
- The 24-well Transwell format enables the these cells to be included in standard drug transporter protocols
- Human assay with no interference from animal inhibitors
- The 21-day cell culture period is reduced to 6-days for customers, saving valuable resources
品質
Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.
法的情報
This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License
関連製品
製品番号
詳細
価格
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MTOX1000CC24-BULK:
MTOX1000CC24-1EA:
MTOX1000CC24-VAR:
最新バージョンのいずれかを選択してください：
Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
資料
腸管排出系トランスポーターと薬物吸収に関する論文を報告しました。
We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.
臨床試験や規制当局への申請の前に、低分子薬物化合物をスクリーニングするために、Caco-2 細胞ベースのアッセイツールを活用します。
Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.
アクティブなフィルタ
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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