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Merck
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MTOX1006P24

Sigma-Aldrich

BCRP/MRP2 Double Knockout Caco-2 Cells

human cervix, Epithelial

別名:

BCRP/MRP2 Double Knockout C2BBe1 Cells

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About This Item

UNSPSCコード:
41106514
NACRES:
NA.81

製品名

BCRP/MRP2 Double Knockout Caco-2 Cells, one assay ready, 24 well plate

由来生物

human colon

フォーム

solid

形態

Epthelial

テクニック

drug transporter assay: suitable
permeability assay: suitable

アプリケーション

ADME/TOX

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詳細

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP/MRP2 double knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the BCRP and MRP2 efflux transporters.

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.

アプリケーション

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

特徴および利点

The Caco-2 subclone, C2BBe1 cells, are ideal for transporter analysis as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:

  • Functional knockout of the BCRP and MRP2 genes eliminates the reliance on chemical inhibitors to determine if a compound is a BCRP or MRP2 substrate
  • The 24 well Transwell format enables the BCRP and MRP2 knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

保管分類コード

13 - Non Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MTOX1006P24-1EA:
MTOX1006P24-VAR:
MTOX1006P24-BULK:

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Mark I Kaldas et al.
The Journal of pharmacy and pharmacology, 55(3), 307-312 (2003-05-02)
Resveratrol is a dietary constituent suggested to have protective effects against cancer as well as cardiovascular disease. The purpose of the study was to learn whether this agent could be absorbed in man and enter the systemic circulation. This was
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
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資料

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

薬物吸収がより予測的な Caco-2 腸管薬物トランスポーターモデル

臨床試験や規制当局への申請の前に、低分子薬物化合物をスクリーニングするために、Caco-2 細胞ベースのアッセイツールを活用します。

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

関連コンテンツ

創薬におけるターゲット・リード・安全性スクリーニングのための必需品

創薬ターゲットとリードの特定に向けた確実なスクリーニングには、試験を経てプロセスに適合したハイスループット対応の試薬と機器が必要です。CRO、製薬企業またはバイオ企業における低分子および生物製剤のスクリーニングプロセス、ならびに治療安全性のための厳格な免疫原性試験に適したツールを選びましょう。

Lead Safety Screening for Drug Discovery

High-throughput reagents for drug target screening and immunogenicity testing.

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