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UFC8003

Millipore

Amicon® Ultra Centrifugal Filter, 3 kDa MWCO

greener alternative

sample volume 4 mL, regenerated cellulose membrane, MWCO 3 kDa

別名:

Centrifugal concentrator, 3 kDa MWCO, 4 mL sample volume

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About This Item

UNSPSCコード:
41104916
eCl@ss:
36100101
NACRES:
NB.24
This item can only be ordered on the Merck Millipore website.

物質

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

品質水準

400

無菌性

non-sterile

製品種目

Amicon®

包装

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

メーカー／製品名

Amicon® Ultra

sustainability

Greener Alternative Product

パラメーター

4 mL sample volume

テクニック

protein extraction: suitable
protein purification: suitable

L

124 mm (4.88 in.)

直径

17.3 mm

ろ過面積

3 cm2

容量

4 mL

有効容積

≤4 mL
4 mL

ポアサイズ

3 kDa MWCO

環境により配慮した代替製品カテゴリ

Re-engineered

輸送温度

ambient

関連するカテゴリー

詳細

Filter Code: PLBC
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

アプリケーション

  • Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.
  • Size exclusion and protein fractionation.

特徴および利点

Minimum Final Concentrate Volume: 50 µL

物理的形状

Color Code: 透明　ピンク色キャップ付

法的情報

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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1 of 3

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資料

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

ナノ粒子の限外ろ過

ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

タンパク質サンプルの限外ろ過

溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。

プロトコル

限外ろ過よる簡単なタンパク質濃縮法

ヒト血漿プロテオームは、バイオマーカーの発見と検討を行うための豊富な資源です。

A Simple Strategy for Protein Enrichment Using Ultrafiltration

The human plasma proteome is a vast resource for biomarker discovery and investigation.

質問

1–3/3 質問  

  1. What could be the reason for no protein recovery (in the concentrate or flow through) while using the UFC800324 to purify protein? Could the protein may be absorbed in the filter?

    1 回答

    1. The low or no protein recovery issue may be attributed to over-concentration of the protein, leading to precipitation. The User Guide provides guidance on maximizing sample recovery: Low sample recovery in the concentrate may be due to adsorptive losses, over-concentration, or passage of sample through the membrane. Adsorptive losses depend upon solute concentration, its hydrophobic nature, temperature and time of contact with filter device surfaces, sample composition, and pH. To minimize losses, remove concentrated samples immediately after centrifugal spin. If the starting sample concentration is high, monitor the centrifugation process in order to avoid over-concentration of the sample. Over-concentration can lead to precipitation and potential sample loss. If the sample appears to be passing through the membrane, choose a lower MWCO Amicon device.

      役に立ちましたか？

  2. What is max g force these filters can take? i.e. what sort of rpm or g force should I spin at?

    1 回答

    1. The UFC8003 protocol states to spin at 7,500 × g maximum for approximately 10–40 minutes. See the image below for further details.

      役に立ちましたか？

  3. Can we centrifuge these tubes in a normal 15 mL swinging bucket centrifuge?

    1 回答

    1. Yes, the device can be used in centrifuges with a swinging bucket or fixed-angle rotor.

      役に立ちましたか？

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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