There is no maximum protein load for these devices, and optimal protein concentrations must be determined empirically. We do recommend a clarifying spin prior to filtration to bring down larger particulates.
This item can only be ordered on the Merck Millipore website.
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物質
polyethylene cap
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品質水準
無菌性
non-sterile
製品種目
Amicon®
包装
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
メーカー／製品名
Amicon® Ultra
sustainability
Greener Alternative Product
パラメーター
4 mL sample volume
テクニック
protein extraction: suitable
protein purification: suitable
L
124 mm (4.88 in.)
直径
17.3 mm
ろ過面積
3 cm2
容量
4 mL
有効容積
≤4 mL
4 mL
ポアサイズ
100 kDa MWCO
環境により配慮した代替製品カテゴリ
輸送温度
ambient
詳細
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
アプリケーション
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
特徴および利点
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
法的情報
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10-13 - German Storage Class 10 to 13
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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資料
Filtration methods and membrane filter selection in nanoparticle purification and production.
ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。
プロトコル
ヒト血漿プロテオームは、バイオマーカーの発見と検討を行うための豊富な資源です。
The human plasma proteome is a vast resource for biomarker discovery and investigation.
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What is the maximum recommended starting protein concentration from Vero cell lysate to avoid overwhelming the filter?
1 回答-
役に立ちましたか？
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Can I use Amicon Ultra 100 kDa filter only for protein sample filtration, to avoid concentration or sample purification?
1 回答-
This device is intended for use as a protein concentrator. Proteins above 100 kDa will be retained while smaller proteins will pass through to the filtrate fraction. Other particles within the sample may be present in both the filtrate and the retentate, depending on the nature of the particle, the nature of the filter membrane, and the centrifugation protocol or technique used. To discuss this further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
役に立ちましたか？
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Les Amicons sont-il RNase/DNase free- DNA/RNA free? Traitement à l'oxyde d'éthylène?
1 回答-
The Amicon products are not tested for RNase/DNase activity. These items are not considered RNase/DNase free. They are not ethylene oxide treated.
役に立ちましたか？
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アクティブなフィルタ
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