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安全性情報

SCT117

Sigma-Aldrich

BioTracker NIR694 Nuclear Dye (Water)

Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.

別名:

Live cell imaging probe

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About This Item

UNSPSCコード:
12352207
NACRES:
NA.47
現在、価格および在庫状況を閲覧できません。

テクニック

cell based assay: suitable

検出方法

fluorometric

輸送温度

ambient

関連するカテゴリー

詳細

The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.

The BioTracker NIR694 Nuclear Dye (Water) is a far red nuclear stain for live cells. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. The dye specifically stains the nucleus of live cells. Staining does not require a wash step and demonstrates greater photostability than the traditional blue fluorescent nuclear stains DAPI and Hoechst. also can be used to stain live cells for cell cycle distribution analysis by flow cytometry BioTracker NIR694 Nuclear Dye (Water) staining is not fixable. For fixed cells we recommend the BioTracker NIR694 Nuclear Dye (DMSO).

Spectral Properties
Absorbance: 662nm
Emission: 694nm

アプリケーション

Live cell fluorescent imaging
Live cell imaging far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

品質

Spectral Properties
Absorbance: 662nm
Emission: 694nm

物理的形状

Liquid

保管および安定性

Store BioTracker NIR694 Nuclear Dye (Water) at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

does not flash

引火点(℃)

does not flash

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SCT117:

試験成績書（COA）

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質問

1–2/2 質問  

  1. The data sheet indicates to dilute BioTracker™ NIR694 Nuclear Dye in cell culture medium or PBS to a final concentration of 1X. However, it does not specify the starting concentration, leaving uncertainty about the appropriate dilution ratio. Can you provide guidance on the starting concentration and the amount needed for dilution?

    1 回答

    1. The information provided by the supplier for SCT117 indicates that the product is at a 200x concentration. To perform staining of fixed and permeabilized cells for cell number normalization, it is recommended to conduct a standard curve of cell density and test RedDot™1 at 1X and 2X final concentration in order to determine the optimal staining concentration for achieving a linear fluorescence signal for your specific cell type.

      役に立ちましたか？

  2. What is the stock concentration of this product? We purchased it recently, but it doesn’t say on the website or the product documentation. 

    1 回答

    1. SCT117 is provided at a 200X concentration.

      役に立ちましたか？

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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