Hoechst 33342 is commonly used for live-cell imaging. Fixation is not required as this material is more lipophilic as compared to the Hoechst 33258. This product has not been tested specifically on oocytes, however several publication referencing this material were found in a literature search. Please see the link below to review one example:
Effect of Hoechst 33342 staining on developmental competence of prepubertal goat oocytes
https://pubmed.ncbi.nlm.nih.gov/12214800/
14533
ビスベンズイミド H 33342 三塩酸塩
suitable for fluorescence, ≥97.0% (HPLC)
別名:
2′-(4-エトキシフェニル)-5-(4-メチル-1-ピペラジニル)-2,5′-ビ-1H-ベンゾイミダゾール 三塩酸塩, HOE 33342, Hoechst 33342, ビスベンズイミド
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| あなたへ/カタログ番号 | 在庫状況 | 単価 |
|---|---|---|
100 mg | カートの在庫状況を確認する | ￥35,600 |
この商品について
実験式（ヒル表記法）:
C27H28N6O · 3HCl · xH2O
CAS番号:
分子量:
561.93 (anhydrous basis)
UNSPSC Code:
12161505
NACRES:
NA.25
PubChem Substance ID:
EC Number:
245-691-1
Beilstein/REAXYS Number:
1234011
MDL number:
Quality Segment
assay
≥97.0% (HPLC)
fluorescence
λex 340 nm; λem 510 nm, λex 355 nm; λem 465 nm in TE buffer; DNA
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Cl[H].CCOc1ccc(cc1)C2=NCc3cc(ccc3N2)C4=NCc5cc(ccc5N4)N6CCN(C)CC6
InChI
1S/C29H32N6O.3ClH/c1-3-36-25-8-4-20(5-9-25)28-30-18-22-16-21(6-10-26(22)32-28)29-31-19-23-17-24(7-11-27(23)33-29)35-14-12-34(2)13-15-35;;;/h4-11,16-17H,3,12-15,18-19H2,1-2H3,(H,30,32)(H,31,33);3*1H
InChI key
FYEVKHPLBHLWHK-UHFFFAOYSA-N
Application
DNA、染色体および核の染色に有用です。蛍光顕微鏡およびフロ-サイトメトリ-に使用可能です。
Excitation max. = 346 nm
Emission max. = 460 nm
Excitation max. = 346 nm
Emission max. = 460 nm
ビスベンズイミドH 33342三塩酸塩は、生化学物質および試薬、蛍光プローブ、ラベル、粒子および染色、発光物質、検出および波長の指標を検討する際に使用可能です。ビスベンズイミドH 33342三塩酸塩、Hoechst 33342は、ヒトTリンパ芽球性白血病細胞に対する無機および有機ヒ素化合物の影響を調査する研究で使用されています。Hoechst 33342は、HeLa細胞およびABCG2 / BCRPを過剰発現するSN-38耐性HeLa（HeLa / SN100）細胞においてABCG2 / BCRPを介した耐性と輸送に対する9種類のカルシウム拮抗薬の効果を調べる研究でも使用されています。
Biochem/physiol Actions
DNAのA-T領域にインタ-カレ-トされる、低細胞毒性の膜透過性蛍光DNA染色色素です。
Packaging
底の開いたガラス瓶内容物は内部に挿入され接着された円錐部に入っています。
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当該品目 | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability - |
| fluorescence λex 340 nm; λem 510 nm, λex 355 nm; λem 465 nm in TE buffer; DNA | fluorescence - | fluorescence - | fluorescence λex 346 nm, λem 460 nm |
| assay ≥97.0% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
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保管分類
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Is it necessary to fix and permeabilize to use hoechst with oocytes?
1 回答-
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How can I determine the shelf life / expiration / retest date of this product?
1 回答-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf役に立ちましたか？
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 回答-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the Department of Transportation shipping information for this product?
1 回答-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
役に立ちましたか？
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What is the solubility of Product 14533, bisBenzimide H 33342 trihydrochloride?
1 回答-
It is soluble in water at 50 mg/mL. It will precipitate from phosphate buffer solutions
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When Product 14533, bisBenzimide H 33342 trihydrochloride, is dissolved in water, what is the pH?
1 回答-
The pH of a 2% solution in water is approximately 1.9.
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