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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
DO-7, monoclonal
フォーム
liquid
含まれません
preservative
交差性
bovine, monkey, human
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
アイソタイプ
IgG2b
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... TP53(7157)
詳細
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.
This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).
免疫原
Epitope: within amino acids 37-45 of p53
recombinant, human p53 expressed in E. coli
アプリケーション
Frozen Sections (1-2 g/ml)
Immunoblotting (1-2 g/ml)
Immunofluorescence (0.5 g/ml)
Immunoprecipitation (2 g/mg lysate)
Paraffin Sections (1-2 g/ml)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
アナリシスノート
Positive Control
SKBR-3 or MDA-231 cells or colon carcinoma tissue
SKBR-3 or MDA-231 cells or colon carcinoma tissue
その他情報
Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Lane, D.P. 1992. Nature358, 15.
Lane, D.P. 1992. Nature358, 15.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
11 - Combustible Solids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
OP140-UG:
OP140-100UG:
US1OP140-EACH:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Jinichi Mori et al.
Neoplasia (New York, N.Y.), 19(3), 185-195 (2017-02-06)
p53 activation by cellular stresses induces the transcription of hundreds of its target genes. To elucidate the entire picture of its downstream pathway, we screened a cDNA microarray dataset of adriamycin-treated HCT116 p53-/- or p53+/+ cells and identified EPSIN 3
Takafumi Miyamoto et al.
Science advances, 3(5), e1603204-e1603204 (2017-06-01)
The transcription factor p53 is at the core of a built-in tumor suppression system that responds to varying degrees of stress input and is deregulated in most human cancers. Befitting its role in maintaining cellular fitness and fidelity, p53 regulates
Tomoyuki Koguchi et al.
International journal of oncology, 48(6), 2415-2424 (2016-04-02)
In response to various cellular stresses, p53 exerts its tumor suppressive effects such as apoptosis, cell cycle arrest, and senescence through the induction of its target genes. Recently, p53 was shown to control cellular homeostasis by regulating energy metabolism, glycolysis
Franz Meitinger et al.
The Journal of cell biology, 214(2), 155-166 (2016-07-20)
In normal human cells, centrosome loss induced by centrinone-a specific centrosome duplication inhibitor-leads to irreversible, p53-dependent G1 arrest by an unknown mechanism. A genome-wide CRISPR/Cas9 screen for centrinone resistance identified genes encoding the p53-binding protein 53BP1, the deubiquitinase USP28, and
Matteo Rossi et al.
Nucleic acids research, 36(22), 7168-7180 (2008-11-19)
PPM1D (Wip1), a type PP2C phosphatase, is expressed at low levels in most normal tissues but is overexpressed in several types of cancers. In cells containing wild-type p53, the levels of PPM1D mRNA and protein increase following exposure to genotoxic
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