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Merck
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主要文書

安全性情報

324506

Millipore

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

別名:

EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated, Ethylenediaminetetraacetic Acid Trisodium Salt

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About This Item

実験式(ヒル表記法):
C10H13N2Na3O8 · 3H2O
CAS番号:
分子量:
412.23
MDL番号:
UNSPSCコード:
12352107
NACRES:
NA.25
現在、価格および在庫状況を閲覧できません。

グレード

Molecular Biology

品質水準

フォーム

liquid

反応適合性

reagent type: chelator

メーカー/製品名

Calbiochem®

保管条件

OK to freeze

pH

8

輸送温度

ambient

保管温度

15-25°C

SMILES記法

[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O

InChI

1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3

InChI Key

FXNQQEVEDZAAJM-UHFFFAOYSA-K

詳細

Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.

アプリケーション

  • Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification.: This research compares various extraction methods for palaeoproteomic analysis, including those utilizing EDTA, to identify bone species efficiently. The study underscores the importance of EDTA in enhancing protein extraction for proteomic studies (Mylopotamitaki et al., 2023).

  • Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease.: The use of EDTA in the preparation and extraction of bone samples for proteomic analysis is discussed, emphasizing its role in achieving deep proteomic coverage and facilitating new discoveries in skeletal biology (Rose et al., 2023).

警告

Toxicity: Standard Handling (A)

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ピクトグラム

Health hazardCorrosion

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Eye Dam. 1 - STOT RE 2 Inhalation

ターゲットの組織

Respiratory Tract

保管分類コード

10 - Combustible liquids

WGK

WGK 2


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

324506-ML:
324506-100ML:


試験成績書(COA)

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質問

1–4/4 質問  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 回答
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      役に立ちましたか?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 回答
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      役に立ちましたか?

  3. Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline

    1 回答
    1. Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.

      役に立ちましたか?

  4. Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia

    1 回答
    1. The solution is prepared with DEPC-treated H2O.

      役に立ちましたか?

レビュー

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アクティブなフィルタ

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