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BMS

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT.

• 別名: BMS, bis(2-Mercaptoethylsulfone)
• 実験式（ヒル表記法）: C₄H₁₀O₂S₃
• CAS番号: 145626-87-5
• 分子量: 186.32
• MDL番号: MFCD00467637
• UNSPSCコード: 12352128

特性

• 品質水準: 100
• アッセイ: ≥99% (titration)
• フォーム: crystalline solid
• 反応適合性: reagent type: reductant
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white
• pKa: (1) 7.9, (2) 9.0
• 輸送温度: ambient
• 保管温度: −20°C
• SMILES記法: [S](=O)(=O)(CCS)CCS
• InChI: 1S/C4H10O2S3/c5-9(6,3-1-7)4-2-8/h7-8H,1-4H2
• InChI Key: YWXCBLUCVBSYNJ-UHFFFAOYSA-N

詳細

詳細

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at pH 7 with BMS than with DTT. Has a pK_a of 7.9 and 9.0.

Water-soluble reagent useful for the reduction of native disulfide bonds in proteins. Considered to be a superior reducing agent to DTT. Shown to reduce accessible disulfide bonds in immunoglobulin and trypsinogen under non-denaturing conditions 5 to 7 times faster than DTT at pH 7. The relatively less accessible disulfide bond in α-chymotrypsinogen A was reduced 2.3 times faster at with BMS than with DTT at pH 7.0.

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

調製ノート

May require warming (40-45°C) to achieve complete solubilization in H₂O.

その他情報

Singh, R., et al. 1995. Methods Enzymol.251, 167.
Singh, R., and Whitesides, G.M. 1994. Bioorg. Chem.22, 109.
Singh, R., and Whitesides, G.M. 1994. Protein Sci. 3, Suppl. 1, 430T.
Lamoureux, G.V., and Whitesides, G.M. 1993. J. Org. Chem.58, 633.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable