748110

SF₇-AM

95% (HPLC)

• 別名: SF₇-AM, Bis(acetoxymethyl)
• 実験式（ヒル表記法）: C₃₁H₂₃N₇O₁₂
• CAS番号: 1416872-50-8
• 分子量: 685.55
• UNSPSCコード: 12352200
• PubChem Substance ID: 329765872

特性

• アッセイ: 95% (HPLC)
• mp: 160-165 °C
• 保管温度: −20°C
• SMILES記法: CC(OCOC(CN(CC(OCOC(C)=O)=O)C(C1=CC=C(C(OC23C4=C(C=C(N=[N+]=[N-])C=C4)OC5C2C=CC(N=[N+]=[N-])=C5)=O)C3=C1)=O)=O)=O
• InChI: 1S/C31H25N7O12/c1-16(39)45-14-47-27(41)12-38(13-28(42)48-15-46-17(2)40)29(43)18-3-6-21-24(9-18)31(50-30(21)44)22-7-4-19(34-36-32)10-25(22)49-26-11-20(35-37-33)5-8-23(26)31/h3-11,22,25H,12-15H2,1-2H3
• InChI Key: KVUJKFQKNGAJST-UHFFFAOYSA-N

詳細

アプリケーション

SF₇-AM is a fluorogenic; or "caged" rhodamine 110 derivative for the detection of hydrogen sulfide (H₂S). Upon exposure to H₂S; the azide functional groups are reduced to primary amines; restoring the fluorescence of the "caged" fluorophore. A fluorescence intensity increase of 20x is observed upon 1h of SF₇-AM exposure to H₂S.[1] This activation of SF₇-AM has been shown to detect H₂S levels as low as 500 nM in vitro.[1] SF₇-AM has also been engineered specifically for use in live cell imaging of H₂S through the addition of the acetoxymethyl ester groups; which are cleaved in vivo by esterases. This results in adding a net -2 charge to the probe; which was demonstrated to "trap" the probe in cells; thus increasing the sensitivity of the probe even when the cell culture media was changed.[1]

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H315,H319,H335
• 注意書き: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable