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HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC

HPLC Analysis of Mannose and Maltose Sugars on Ascentis® Express HILIC application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53981-U

Ascentis® Express HILIC HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

M8378

Maltotriosio

≥90% (HPLC)

M7753

Maltoheptaose

≥60% (HPLC)

M9153

Maltohexaose

≥65% (HPLC)

1375025

Maltosio

United States Pharmacopeia (USP) Reference Standard

1375182

D-(+)-mannosio

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (25:75, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Descrizione

Risultati analitici

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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