Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Functionalization of mesoporous silica nanoparticles with a cell-penetrating peptide to target mammalian sperm in vitro.

Nanomedicine (London, England) (2015-05-27)

Natalia Barkalina, Celine Jones, Helen Townley, Kevin Coward

ABSTRACT

This study aimed to investigate the effects of actively targeting mesoporous silica nanoparticles (MSNPs) toward mammalian sperm with a cell-penetrating peptide (C105Y), with subsequent analysis of binding rates and nano-safety profiles. Boar sperm were exposed in vitro to C105Y-functionalized MSNPs or free C105Y, in a series of increasing doses for up to 2 h, followed by the evaluation of sperm motility, kinematic parameters, acrosome morphology, MSNP-sperm binding and cell fluorescence levels. C105Y-functionalized MSNPs preserved their biocompatibility with sperm, and exhibited an approximately fourfold increase in affinity toward gametes, compared with unmodified MSNPs, during the early stages of incubation. Our findings support the application of MSNPs and active targeting to sperm as valuable tools for reproductive biology.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

440140

Sigma-Aldrich

(3-amminopropil)trimetossisilano, 99%

H5882

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, ≥98%

F8775

Sigma-Aldrich

Formaldeide, for molecular biology, 36.5-38% in H₂O

72068

Sigma-Aldrich

Idrossido di sodio, BioUltra, for molecular biology, 10 M in H₂O

H9892

Sigma-Aldrich

Acido cloridrico, 1.0 N, BioReagent, suitable for cell culture

S2770

Sigma-Aldrich

Idrossido di sodio, 1.0 N, BioReagent, suitable for cell culture

H6269

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, for molecular biology, ≥99%

H1758

Sigma-Aldrich

Acido cloridrico, 36.5-38.0%, BioReagent, for molecular biology

2104

Supelco

Acido cloridrico, volumetric, 0.1 M HCl (0.1N), endotoxin free

H9151

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, BioXtra, ≥99%

47608

Sigma-Aldrich

Formaldeide, for molecular biology, BioReagent, ≥36.0% in H₂O (T)

741442

Sigma-Aldrich

(3-amminopropil)trimetossisilano, ≥98.0%

45726

Sigma-Aldrich

Acido acetico, for luminescence, BioUltra, ≥99.5% (GC)

52365

Sigma-Aldrich

Esadeciltrimetilammonio bromuro, BioUltra, for molecular biology, ≥99.0% (AT)

W200611

Sigma-Aldrich

Acido acetico, ≥99.5%, FCC, FG

252549

Sigma-Aldrich

Formaldeide, ACS reagent, 37 wt. % in H₂O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

71687

Sigma-Aldrich

Sodio idrossido, BioUltra, for luminescence, ≥98.0% (T), pellets

84429

Sigma-Aldrich

Acido cloridrico, ~6 M in H₂O, for amino acid analysis

W200603

Sigma-Aldrich

Acido acetico, natural, ≥99.5%, FG

A8380

Sigma-Aldrich

5α-Androstan-17β-ol-3-one, ≥97.5%

719803

Sigma-Aldrich

Carbon nanofibers, graphitized, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm

705551

Sigma-Aldrich

Cloruro di idrogeno, 3 M in cyclopentyl methyl ether (CPME)

151173

Sigma-Aldrich

Ninhydrin, ACS reagent

719811

Sigma-Aldrich

Carbon nanofibers, pyrolitically stripped, platelets(conical), >98% carbon basis, D × L 100 nm × 20-200 μm

284505

Sigma-Aldrich

1,2-dicloroetano, anhydrous, 99.8%

699632

Sigma-Aldrich

Carbon, mesoporous, nanopowder, less than 500 ppm Al, Ti, Fe, Ni, Cu, and Zn combined

W530574

Sigma-Aldrich

Acido cloridrico, 32 wt. % in H₂O, FCC

757527

Sigma-Aldrich

Sodio idrossido, ultra dry, powder or crystals, 99.99% trace metals basis

699624

Sigma-Aldrich

Graphite, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined

699640

Sigma-Aldrich

Carbon, mesoporous, less than 100 ppm Al, Ti, Fe, Ni, Cu, and Zn combined