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Merck
  • Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography.

Facile preparation of polyvinyl alcohol coated SiO2 stationary phases for high performance liquid chromatography.

The Analyst (2014-09-03)
Shunli Ji, Feifang Zhang, Shengjie Wu, Bingcheng Yang, Xinmiao Liang
ABSTRACT

A facile method to prepare a polar stationary phase for hydrophilic interaction chromatography (HILIC) was proposed by coating polyvinyl alcohol onto silica particles (PVA-Sil). A water or organic solvent-insoluble permanent PVA coating on the silica particle surface can be formed simply by dipping silica particles into a hot PVA solution and then settled from this solution, leaving a thin layer of PVA coating and frozen in a freezer. The PVA-Sil shields the silica core from solution erosion to some degree and the pH tolerance range was extended to 9.5 from 8.0 for bare silica. PVA-Sil demonstrated a good hydrophilic property for several kinds of polar compounds and ∼57000 m(-1) of plate count was achieved. This method can also be extended as a universal method to prepare various stationary phases with exchangeable functionalities by doping the desired ingredient in a PVA solution.

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Sigma-Aldrich
Acido formico, reagent grade, ≥95%
Sigma-Aldrich
Acido formico, ACS reagent, ≥96%
Sigma-Aldrich
Formato di ammonio, reagent grade, 97%
Sigma-Aldrich
Formato di ammonio, ≥99.995% trace metals basis
Sigma-Aldrich
Acido formico, ACS reagent, ≥88%
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Ammonium formate solution, BioUltra, 10 M in H2O
Sigma-Aldrich
Acido formico, ≥95%, FCC, FG
Supelco
Formato di ammonio, eluent additive for LC-MS, LiChropur, ≥99.0%
Sigma-Aldrich
Formic acid solution, BioUltra, 1.0 M in H2O
Sigma-Aldrich
Formato di ammonio, BioUltra, ≥99.0% (calc. based on dry substance, NT)