BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

Antiviral research (2014-12-03)

Arul Balaji Velu, Guang-Wu Chen, Po-Ting Hsieh, Jim-Tong Horng, John Tsu-An Hsu, Hsing-Pang Hsieh, Tzu-Chun Chen, Kuo-Feng Weng, Shin-Ru Shih

PMID25448086

ABSTRACT

Enterovirus 71 (EV71) infections can cause hand, foot, and mouth disease with severe neurological complications. Because no clinical drug is available for treating EV71 infections, developing an efficient antiviral medication against EV71 infection is crucial. This study indicated that 6-bromo-2-[1-(2,5-dimethylphenyl)-5-methyl-1H-pyrazol-4-yl] quinoline-4-carboxylic acid (BPR-3P0128) exhibits excellent antiviral activity against EV71 (EC50 = 0.0029 μM). BPR-3P0128 inhibits viral replication during the early post infection stage, targets EV71 RNA-dependent RNA polymerase and VPg uridylylation, and also reduces viral RNA accumulation levels and inhibits viral replication of EV71.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

L3771

Sigma-Aldrich

Sodio dodecil solfato, BioReagent, suitable for electrophoresis, ≥98.5% (GC)

75746

Sigma-Aldrich

Sodio dodecil solfato, ≥99.0% (GC), dust-free pellets

S3014

Sigma-Aldrich

Sodio cloruro, DNase, RNase, and protease, none detected, ≥99% (titration)

S8776

Sigma-Aldrich

Sodio cloruro, 0.9% in water, BioXtra, suitable for cell culture

71736

Sigma-Aldrich

Sodio dodecil solfato, BioUltra, 10% in H₂O

S5886

Sigma-Aldrich

Sodio cloruro, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S5150

Sigma-Aldrich

Sodio cloruro, 5 M in H₂O, BioReagent, suitable for cell culture

59222C

SAFC

Sodio cloruro, 5 M

05030

Sigma-Aldrich

Sodio dodecil solfato, BioUltra, 20% in H₂O

71725

Sigma-Aldrich

Sodio dodecil solfato, BioUltra, ≥99.0% (GC)

P9625

Sigma-Aldrich

Phenazine methosulfate

71386

Sigma-Aldrich

Sodio cloruro, BioUltra, ~5 M in H₂O

204439

Sigma-Aldrich

Sodio cloruro, 99.999% trace metals basis

71376

Sigma-Aldrich

Sodio cloruro, BioUltra, ≥99.5% (AT)

74255

Supelco

Sodio dodecil solfato, dust-free pellets, suitable for electrophoresis, ≥99.0% (GC)

71729

Sigma-Aldrich

Sodio dodecil solfato, ≥98.0% (GC)

436143

Sigma-Aldrich

Sodio dodecil solfato, ACS reagent, ≥99.0%

S7653

Sigma-Aldrich

Sodio cloruro, BioXtra, ≥99.5% (AT)

M0375

Sigma-Aldrich

Acido maleico, ReagentPlus^®, ≥99% (HPLC)

1374500

USP

Acido maleico, United States Pharmacopeia (USP) Reference Standard

L4509

Sigma-Aldrich

Sodio dodecil solfato, ReagentPlus^®, ≥98.5% (GC)

S6191

Sigma-Aldrich

Sodio cloruro, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture

71717

Sigma-Aldrich

Sodio dodecil solfato, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

450006

Sigma-Aldrich

Sodio cloruro, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

451940

Sigma-Aldrich

Sodium chloride-³⁵Cl, 99 atom % ³⁵Cl

S1679

Sigma-Aldrich

Sodio cloruro, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

S6546

Sigma-Aldrich

Sodio cloruro, 5 M

378860

Sigma-Aldrich

Sodio cloruro, random crystals, optical grade, 99.9% trace metals basis

L6026

Sigma-Aldrich

Sodio dodecil solfato, BioXtra, ≥99.0% (GC)

L5750

Sigma-Aldrich

Sodio dodecil solfato, 92.5-100.5% based on total alkyl sulfate content basis