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W1878

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Williams′ Medium E

With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Produzione farmaceutica

Sinonimo/i:

Williams’ E medium

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

product name

Williams′ Medium E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Livello qualitativo

400

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Descrizione generale

Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.

Applicazioni

Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.

Ricostituzione

Supplement with 0.292 g/L L-glutamine.

Altre note

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
Balakrishnan Chakrapani Narmada et al.
Stem cells translational medicine, 6(3), 851-863 (2017-03-16)
Atherosclerosis underlies many cardiovascular and cerebrovascular diseases. Nutraceuticals are emerging as a therapeutic moiety for restoring vascular health. Unlike small-molecule drugs, the complexity of ingredients in nutraceuticals often confounds evaluation of their efficacy in preclinical evaluation. It is recognized that
Ezgi Eyluel Bankoglu et al.
PloS one, 11(11), e0166956-e0166956 (2016-11-29)
Type 2 diabetes (T2DM) and obesity are frequently associated with non-alcoholic fatty liver disease (NAFLD) and with an elevated cancer incidence. The molecular mechanisms of carcinogenesis in this context are only partially understood. High blood insulin levels are typical in
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