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K3753

Sigma-Aldrich

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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About This Item

Codice UNSPSC:
12352207

Livello qualitativo

Forma fisica

powder

tecniche

cell culture | mammalian: suitable

Temperatura di conservazione

2-8°C

Descrizione generale

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Applicazioni

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
  • to collect the uterine and forelimb blood vessel samples from horses

Quantità

Formulated to contain 9.6 grams of powder per liter of medium.

Ricostituzione

Supplement with 2.1 g/L sodium bicarbonate.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Protocolli

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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