SRP6556

Thrombin Active (High Activity) from bovine plasma

≥98% (SDS-PAGE), recombinant, lyophilized

• Sinonimo/i: Activated Factor IIa
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Nome del prodotto: Thrombin Active (High Activity) from bovine plasma, ≥98% (SDS-PAGE)
• Origine biologica: bovine plasma
• Saggio: ≥98% (SDS-PAGE)
• Stato: lyophilized
• Potenza: >1500 units/mg
• PM: 37 kDa
• Confezionamento: pkg of 10,000 units; pkg of 100,000 units; pkg of 1000 units
• N° accesso UniProt: P00735
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: bovine ... F2(280685)

Descrizione

Descrizione generale

Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions.[1]

Azioni biochim/fisiol

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands.[2] Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII.[1] Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet.[3] As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs.[4] After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Thrombin enzyme (Activated Factor IIa) also participates in inflammation and has mitogenic role on vascular cells.[5] It is also involved in the activation of protein C which is needed for the inactivation of many procoagulant enzymes.[6]

Stato fisico

Sterile filtered and lyophilized with Mannitol and Sodium Chloride.

Ricostituzione

Reconstitute in sterile water (100 U/mL) with 0.9% NaCl. It forms a clear solution.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable