SRP3312

IL-10 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Sinonimo/i: B-TCGF, CSIF, TGIF
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forma fisica: lyophilized
• Potenza: ≤2.0 ng/mL
• PM: 18.6 kDa
• Confezionamento: pkg of 10 μg
• Impurezze: endotoxin, tested
• N° accesso UniProt: P22301
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... IL10(3586)

Descrizione

Descrizione generale

Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. The gene encoding this protein is localized on human chromosome 1q31-32. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues. Interleukin 10 (IL-10) comprises interferon IFN-γ fold and exists as a symmetric homodimer. It shares structural similarity with interferon γ (IFN-γ).

Applicazioni

Interleukin 10 (IL-10) human has been used in enzyme-linked immunosorbent assay.

Azioni biochim/fisiol

Interleukin-10 (IL-10) is an anti-inflammatory protein that inhibits the expression of proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α). Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. It inhibits the production of IFN-γ and antigen-specific proliferation of type-1 T helper cells (Th1). It protects the host tissue from damage during immune responses. The protein has a role in modulating viral immunity. IL-10 enhances cytokine production, natural killer cell proliferation and cytotoxicity in vitro. IL-10 is an immunosuppressive cytokine. It promotes wound repair and functions to resolve inflammation in the central nervous system. IL-10 mediates its signaling function through the interleukin receptors namely, IL-10RA and two IL-10RBs. The expression of IL-10 is regulated by signal transducer and activator of transcription 3 (STAT3) and suppressor of cytokine signaling-3 (SOCS3) ratios. Polymorphisms in the IL-10 gene is implicated in breast cancer.

Stato fisico

Lyophilized from 20 mM Sodium Phosphate, pH 7.2.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable