After reconstituting the protein in water, it remains active and stable at 2-8°C for up to a week. However, for long-term stabilization at -20 to -80°C, further dilution with a buffer containing a carrier is necessary to prevent activity loss. It is recommended to use 5% trehalose or 0.1% BSA in water or PBS for extended storage. Working aliquots should have a minimum volume of 10 uL and a concentration above 1 ug/ml for optimal stability.
SRP3027
EGF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinonimo/i:
Epidermal Growth Factor, URG, Urogastrone
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500 μG
428,00 €
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Cambia visualizzazione
500 μG
428,00 €
About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in E. coli
Saggio
≥98% (HPLC)
≥98% (SDS-PAGE)
Stato
lyophilized
Potenza
≤0.2 ng/mL
PM
6.2 kDa
Confezionamento
pkg of 500 μg
tecniche
cell culture | mammalian: suitable
Impurezze
<0.1 EU/μg endotoxin, tested
Colore
white to off-white
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... EGF(1950)
Descrizione generale
The gene EGF (epidermal growth factor) is mapped to human chromosome 4q25-27.[1] The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. The mRNA is expressed in kidney, salivary gland, cerebrum and prostate.[2] Recombinant human EGF is a 6.2kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.
Applicazioni
EGF (epidermal growth factor) human has been used in the culture medium for the maintenance of MCF10A cells (human mammary epithelial cells).[2]
Azioni biochim/fisiol
EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[3] Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing.[4][5] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[6] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). Mutation in the EGF gene might be associated with risk of non- small cell lung adenocarcinoma.[1] It is also involved in anaplastic thyroid cancer and renal hypomagnesemia. It controls cell differentiation and neurotrophic processes.[7][8]
Sequenza
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Stato fisico
Lyophilized with no additives.
Ricostituzione
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
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Is it necessary to dilute item no: SRP3027-500UG in a buffer with a stabilizer for extended storage as mentioned in the reconstitution portion of the specification. If so, would D-(+)-Trehalose dihydrate (cat#T0167) be appropriate? Also, specify the buffer considering that water is indicated as an appropriate diluent in the specifications.
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