SEP050
Seppro® Supermix
LC2
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
Prodotti consigliati
Volume del letto
2 mL
Diam. × lungh. colonna
6.4 mm × 63 mm
Capacità
loading(Flow Through volume from IgY 14 LC5 (SEP030))
Temperatura di conservazione
2-8°C
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Descrizione generale
Immunoaffinity fractionation of highly-abundant proteins (HAP) has proven to be one of the most effective approaches for overcoming the wide dynamic range of protein concentration, as well as enabling the detection of low abundant proteins (LAP). Avian polyclonal IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that allow for highly-specific fractionation of a complex protein mixture. IgY12 column can effectively remove 12 HAP from plasma. The next level of abundant protein, moderately abundant proteins (MAP), becomes an obstacle to access LAP, where the majority of biologically interesting and clinically important biomarkers reside. Therefore, isolation of MAP is a new challenge for effectively and accurately detecting and analyzing LAP.
To tackle this challenge, we further developed our IgY columns system by establishing a SuperMix column to separate MAP from LAP. The SuperMix System was developed by immunizing chickens with a flow-through fraction of IgY12 or IgY14 column and constructing the column with affinity-purified IgY antibodies against the flow-through proteins of IgY12 or IgY14.
To tackle this challenge, we further developed our IgY columns system by establishing a SuperMix column to separate MAP from LAP. The SuperMix System was developed by immunizing chickens with a flow-through fraction of IgY12 or IgY14 column and constructing the column with affinity-purified IgY antibodies against the flow-through proteins of IgY12 or IgY14.
Applicazioni
Seppro® Supermix, LC2 (2mL column) is used to separate moderately abundant proteins (MAP) from human serum low abundant proteins (LAP) after specific human serum highly-abundant proteins (HAP) have been removed using Seppro® IgY12 or IgY14 affinity separation.
Altre note
for removal of moderately abundant proteins from human serum or plasma
Stoccaggio e stabilità
Store the column at 2-8 °C. After use, equilibrate the column with 1′ Dilution Buffer containing 0.02% sodium azide and store the column at 2-8 °C with the end-caps tightly sealed. Do Not Freeze the column.
Note legali
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
Codice della classe di stoccaggio
10 - Combustible liquids
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Effect of plasma protein depletion on BNP-32 recovery.
Adam M Hawkridge et al.
Clinical chemistry, 54(5), 933-934 (2008-04-30)
Does plasma protein depletion increase lung liquid conductance?
P Dodek
American journal of respiratory and critical care medicine, 154(4 Pt 1), 1213-1215 (1996-10-01)
Majlinda Kullolli et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant
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