Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10. Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions , and interference with cell cycle processes of host cells . Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection. It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins.
Immunogeno
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 350-400 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
Applicazioni
SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. Antibody can also be used for as capture antibody in Sandwich Elisa at 10 μg/mL. All other applications and species not yet tested.
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