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RAB0082

Sigma-Aldrich

Human CD163 ELISA Kit

for serum, plasma, cell culture supernatant and urine

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1 KIT
640,00 €

640,00 €


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Cambia visualizzazione
1 KIT
640,00 €

About This Item

Codice UNSPSC:
41116158
NACRES:
NA.32

640,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Reattività contro le specie

human

Confezionamento

kit of 96 wells (12 strips x 8 wells)

tecniche

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 32.77-8000 pg/mL

Metodo di rivelazione

colorimetric

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CD163(9332)

Descrizione generale

The Human CD163 (also known as hemoglobin scavenger receptor) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD163 in serum, plasma, cell culture supernatants and urine.

Immunogeno

Recombinant Human CD163

Applicazioni

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human CD163 ELISA kit has been used to measure the levels of CD163 in plasma samples and cell culture supernatants.[1]

Azioni biochim/fisiol

Cluster of differentiation 163 (CD163) is a scavenger receptor. It is expressed on the surface of activated macrophages. This protein modulates anti-inflammatory functions and has a role in hemoglobin-haptoglobin clearance. Various cytokines influence the expression of CD163. It may be associated with hepatocellular carcinoma.[2][3]

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pittogrammi

Corrosion

Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Met. Corr. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

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A Ross Eckard et al.
HIV medicine, 15(9), 537-546 (2014-03-22)
HIV-infected patients on antiretroviral therapy (ART) have an increased cardiovascular disease (CVD) risk as a result of heightened inflammation and immune activation, despite at times having normal lipids and few traditional risk factors. Biomarkers are needed to identify such patients
Monocyte-lymphocyte cross-communication via soluble CD163 directly links innate immune system activation and adaptive immune system suppression following ischemic stroke
Grant C
Scientific Reports (2017)
Clinical significance of soluble CD163 in polymyositis-related or dermatomyositis-related interstitial lung disease.
Enomoto Y
Arthritis Research & Therapy (2017)
Martin Hoenigl et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 69(4), 676-686 (2018-11-13)
Despite effective antiretroviral therapy (ART), human immunodeficiency virus (HIV) infection remains associated with higher morbidity and mortality, driven, in part, by increased inflammation. Our objective was to identify associations between levels of plasma biomarkers of chronic inflammation, microbial translocation, and
Macrophage activation marker soluble CD163 may predict disease progression in hepatocellular carcinoma.
Kazankov K
Scandinavian Journal of Clinical and Laboratory Investigation (2016)

Domande

1–2 di 2 domande  
  1. Does the CD163 ELISA test for the soluble domain?

    1 risposta
    1. Epitope mapping has not been conducted. The capture and detection antibodies are Monoclonal Mouse IgG1. The immunogen used is mouse myeloma cell line NS0-derived recombinant human CD163 (aa41-1045), with the accession number CAA80543.

      Utile?

  2. Is it possible to analyze plasma containing EDTA and cerebrospinal fluid (CSF) using the RAB0082?

    1 risposta
    1. According to the supplier, Ray Biotech, plasma samples containing EDTA are not suitable for use in this assay due to its chelating property, as noted in the kit manual. Additionally, the assay has not been directly tested with cerebrospinal fluid (CSF), so its compatibility with CSF cannot be confirmed.

      Utile?

Recensioni

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