RNA Marker sample solutions for electrophoresis should be prepared as follows: Mix 2-4 µl of RNA Marker, add water (Rnase free) to make up to 5 µl, then add 3 µl of RNA Sample Loading Buffer, and 1 µl of 200 mM Potassium Acetate, pH 4.5. Additional information can be found in the Product Information Sheet.
R7020
Transcript RNA Markers 0.2-10 kb
for RNA electrophoresis
Sinonimo/i:
RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker
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50 μG
168,00 €
168,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
50 μG
168,00 €
About This Item
Codice UNSPSC:
41105337
NACRES:
NA.25
168,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Grado
Molecular Biology
for molecular biology
Livello qualitativo
Stato
liquid
impiego
25 uses
Compatibilità
suitable for electrophoresis (RNA)
Temperatura di conservazione
−70°C
Categorie correlate
Descrizione generale
Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Applicazioni
Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.[1]
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.[1]
Componenti
Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Altre note
Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocolli
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Contenuto correlato
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
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in what proportion should i mix RNA marker and loading dye buffer.
1 risposta-
Utile?
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Can I denaturate it using Glyoxal?
1 risposta-
This product has been tested on 1% agarose gel with formaldehyde. It has not been specifically qualified for use with glyoxal, however there is no reason to suspect that it would not be suitable. See the reference in the link below comparing formaldehyde to glyoxal:
https://link.springer.com/protocol/10.1385/0-89603-127-6:1Utile?
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