The concentration of the DNA marker is not part of the specification and that information is not available. Product DPLUS-1K - DirectLoad™ Plus 1kb DNA Ladder is a comparable marker that may be considered an alternative. DPLUS-1KT is noted as having a DNA concentration of 100 ug/ul. The DNA concentration for individual bands is unavailable.
D7058
DirectLoad™ Wide Range DNA Marker
ready-to-use marker for DNA electrophoresis
Sinonimo/i:
DirectLoad™ marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder, wide range ladder for gel electrophoresis, wide range marker for gel electrophoresis
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1 VIAL
293,00 €
293,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
1 VIAL
293,00 €
About This Item
Codice UNSPSC:
41105335
NACRES:
NA.25
293,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Livello qualitativo
Stato
liquid
impiego
100 uses
Compatibilità
suitable for electrophoresis (DNA)
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Applicazioni
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Caratteristiche e vantaggi
- Ready-to-load
- Easy-to-use
- Popular band sizes
Componenti
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Altre note
Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Note legali
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Prodotti correlati
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Protocolli
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures.
Contenuto correlato
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Could you provide the concentraion of the marker? Idealy band by band so that it can be used as an estimate of the concentration of samples run along side.
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