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PPC1010

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases, aminopeptidase, serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase

Sinonimo/i:

Protease and Phosphatase Inhibitor Mix, Protease and phosphatase inhibitor

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1 ML
250,00 €
5 ML
534,00 €

250,00 €


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Cambia visualizzazione
1 ML
250,00 €
5 ML
534,00 €

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.54
Stato:
DMSO solution
Temperatura di conservazione:
−20°C

250,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Nome del prodotto

Protease and Phosphatase Inhibitor Cocktail,

Stato

DMSO solution

Livello qualitativo

Confezionamento

pkg of 1 ml, 5 ml ×

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Protease and Phosphatase Inhibitor Cocktail is designed to increase the yield of intact and unmodified proteins in cell extracts. Protease and Phosphatase Inhibitor cocktail mixture inhibits serine, cysteine, and acid proteases, as well as aminopeptidase. It also inhibits serine/threonine protein phosphatases and L-isozymes of alkaline phosphatase. The cocktail has been tested on extracts from various animal tissues such as the human placenta and bovine liver and on extracts of A431, CHO, and U937 cells. Protease and Phosphatase Inhibitor Cocktail is supplied as a ready-to-use solution in DMSO.

Applicazioni

Protease and Phosphatase Inhibitor Cocktail has been used as a supplement in radioimmunoprecipitation assay (RIPA) buffer:
  • for cellular protein extraction[1]
  • to lyse purified CD4+ T cells[2]
  • to prepare protein extracts from placental tissues and cells[3]

Protease and Phosphatase Inhibitor Cocktail has been used in protein isolation buffers.[4][5]

Nota sulla preparazione

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 500 mg of protein extracted from tissue or cells. In many cases, the cocktail can be used at a final concentration of 1% (v/v), 1 ml of cocktail solution per 100 ml of extraction buffer.

Altre note

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

188.6 °F

Punto d’infiammabilità (°C)

87 °C


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Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Domande

1–2 di 2 domande  
  1. Does PPC1010 contain EDTA?

    1 risposta
    1. PPC1010 does not contain EDTA

      Utile?

  2. Does these Protease and Phosphatase Inhibitor Cocktail contains any type of metal chelator or EDTA?

    1 risposta
    1. This product does not contains EDTA or other chelators. See the link below to review the product datasheet which includes the cocktail components:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/113/180/ppc1010dat.pdf

      Utile?

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