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MSSAFE

Sigma-Aldrich

Protease and Phosphatase Inhibitor Cocktail

MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.

Sinonimo/i:

Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail

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1 VIAL
83,10 €
5 VIALS
345,00 €

83,10 €


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Cambia visualizzazione
1 VIAL
83,10 €
5 VIALS
345,00 €

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.77
Stato:
powder
Solubilità:
water: 0.1 g/mL, clear, colorless
Temperatura di conservazione:
2-8°C

83,10 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Richiedi un ordine bulk

Nome del prodotto

MS-SAFE Protease and Phosphatase Inhibitor,

Descrizione

for use with mammalian cell and tissue extract, lyophilized powder

Livello qualitativo

Stato

powder

Solubilità

water: 0.1 g/mL, clear, colorless

Temperatura di conservazione

2-8°C

Descrizione generale

Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:
  • Serine, cysteine and aspartic proteases, and metalloproteases
  • Tyrosine, serine/threonine, acid and alkaline phosphatases
All inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment.

Specificità

Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases

Applicazioni

MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.[1]
Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.

Caratteristiche e vantaggi

Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis

Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment

Allows accurate measurement of protein activity and identification of phosphorylation sites.

Componenti

Protease inhibitors:
  • Bestatin hydrochloride
  • Leupeptin
  • Phosphoramidon disodium salt
  • Pepstatin A
  • Elastatinal
  • Aprotinin
  • Nafamostat mesylate
  • Antipain
Phosphatase inhibitors
  • Okadaic acid
  • Sodium fluoride
  • Sodium orthovanadate
  • Bromotetramisole oxalate
Fillers
  • β-lactose
  • DL-leucine

Quantità

One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.

Stato fisico

Lyophilized powder that is water-soluble

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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S K Sreedharan et al.
The Biochemical journal, 316 ( Pt 3), 777-786 (1996-06-15)
1-trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64) was shown to inhibit beta-trypsin by a reversible competitive mechanism; this contrasts with the widely held view that E-64 is a class-specific inhibitor of the cysteine proteinases and reports in the literature that it does not inhibit a
Mark D Schuchard et al.
BioTechniques, 39(2), 239-247 (2005-08-25)
The inclusion of protease inhibitors in serum or plasma samples has been found to significantly impact the isoform profile of selected plasma proteins as seen on 2-dimensional electrophoresis (2-DE) gels. With the addition of a protease inhibitor cocktail, several human
Aberrant REDD1-mTORC1 responses to insulin in skeletal muscle from Type 2 diabetics.
Williamson, D.L., et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 309, R855-R863 (2015)
Junguo Ma et al.
Chemosphere, 194, 773-783 (2017-12-19)
A previous study showed that microcystin-LR (MC-LR) exerted cytotoxicity and induced apoptosis in HepG2 cells. In the present study, we investigated whether oxidative stress-mediated p53/p21
Yumei Li et al.
Oncology letters, 14(6), 8035-8041 (2018-01-19)
Rhabdoid tumors, which tend to occur prior to the age of 2 years, are one of the most aggressive malignancies and have a poor prognosis due to the frequency of metastasis. Silibinin, a natural extract, has been approved as a

Contenuto correlato

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Domande

Recensioni

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