PEROX1
Peroxisome Isolation Kit
isolate peroxisomes from tissues and cells
Sinonimo/i:
Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit
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1 KIT
804,00 €
804,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
1 KIT
804,00 €
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
804,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Livello qualitativo
tecniche
centrifugation: suitable
fractionation: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
Applicazioni
The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.
Caratteristiche e vantaggi
- Specially formulated extraction reagents for research scale applications - save time and minimize waste
- Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
- Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)
Altre note
Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.
Note legali
OptiPrep is a trademark of Serumwerk Bernburg AG
I componenti del kit sono disponibili anche separatamente
N° Catalogo
Descrizione
SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Punto d’infiammabilità (°F)
188.6 °F - closed cup
Punto d’infiammabilità (°C)
87 °C - closed cup
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K Burdett et al.
The Journal of biological chemistry, 266(19), 12201-12206 (1991-07-05)
Upon differential centrifugation of guinea pig intestine mucosal cells homogenate, fatty acyl-CoA:NADPH oxidoreductase (long chain alcohol forming) was found to be enriched in the light mitochondrial (L) fraction (sedimenting between 66,000 x g min and 500,000 x g min) which
M Y Kang et al.
Cell death and differentiation, 20(1), 117-129 (2012-08-25)
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M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
Dieanira Erudaitius et al.
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The high extracellular hydrogen peroxide (H2O2) concentrations generated during pharmacological ascorbate (P-AscH-) therapy has been shown to exhibit a high flux into susceptible cancer cells leading to a decrease in clonogenic survival. It is hypothesized that the intracellular H2O2 concentration
Articoli
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Filtri attivi
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