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N3013

Sigma-Aldrich

Nile Red

Powder

Sinonimo/i:

Nile Blue A Oxazone

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100 MG
185,00 €
1 G
1.100,00 €

185,00 €

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Cambia visualizzazione
100 MG
185,00 €
1 G
1.100,00 €

About This Item

Formula empirica (notazione di Hill):
C20H18N2O2
Numero CAS:
7385-67-3
Peso molecolare:
318.37
Beilstein:
279110
Numero CE:
230-966-0
Numero MDL:
MFCD00011639
Codice UNSPSC:
12171500
ID PubChem:
24897612
NACRES:
NA.47

185,00 €

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Nome del prodotto

Nile Red, Technical grade

Grado

technical grade

Stato

powder

Composizione

Carbon: 70.0-77.3%

Nitrogen: 7.5-8.8%

tecniche

titration: suitable

Colore

faint green to brown

Punto di fusione

203-205 °C (lit.)

Solubilità

methanol: 1 mg/mL

λmax

553 nm

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34

InChI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
VOFUROIFQGPCGE-UHFFFAOYSA-N

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Descrizione generale

Nile Red is a lipophilic fluorescent dye that is commonly used in various scientific applications, particularly in lipid research. It has the ability to stain and visualize lipid droplets in cells and tissues.

Applicazioni

Nile red is widely employed in studies related to lipid metabolism, lipid droplet dynamics, lipid accumulation in cells or tissues, and lipid-based drug delivery systems.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLBP9326V
070K3462
063K3730
063K3691
061K3444

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D L Sackett et al.
Analytical biochemistry, 167(2), 228-234 (1987-12-01)
Nile red is an uncharged hydrophobic molecule whose fluorescence is strongly influenced by the polarity of its environment. It interacts with many, but not all, native proteins, including beta-lactoglobulin, kappa-casein, and albumin, with a wide range of spectral changes for
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Domande

  1. How should n3013 be dissolved and diluted for lipid staining?

    1 risposta

    1. There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
      To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.

      Utile?

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