MAK173
Universal Fluorometric Kinase Assay Kit
sufficient for 250 fluorometric tests
Sinonimo/i:
Universal Kinase Test Kit
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
1 KIT
662,00 €
Scegli un formato
Cambia visualizzazione
1 KIT
662,00 €
About This Item
Prodotti consigliati
impiego
sufficient for 250 fluorometric tests
Metodo di rivelazione
fluorometric
Malattie correlate
endocrinological disorders, diabetes; neurological disorders; cardiovascular diseases; cancer
Temperatura di conservazione
−20°C
Categorie correlate
Descrizione generale
Protein kinases are the enzymes that catalyze the transfer of phosphate group from a phosphate donor to an acceptor in a substrate protein. Kinases are of great interest to researchers due to their role in cell signaling and their roles in disease states.
Most of the commercial protein kinase assay kits are based on monitoring either phosphopeptide formation or the ATP depletion. Kinase assays that rely on the detection of phosphopeptides require significant effort to identify an optimized peptide for the specific kinase of interest. The ATP depletion method suffers various interferences due to inhibition or activation of luciferase by compounds found in the biological matrix.
Most of the commercial protein kinase assay kits are based on monitoring either phosphopeptide formation or the ATP depletion. Kinase assays that rely on the detection of phosphopeptides require significant effort to identify an optimized peptide for the specific kinase of interest. The ATP depletion method suffers various interferences due to inhibition or activation of luciferase by compounds found in the biological matrix.
Compatibilità
Suitable for the determination of kinase activity and for screening and identifying kinase inhibitors
Principio
This Universal Fluorimetric Kinase Assay Kit is based on monitoring ADP formation, which is directly proportional to kinase activity. This enzyme-coupled kit provides a fast, efficient, and homogeneous assay to measure kinase activities. This assay kit is a non-radioactive and non-antibody based method to detect the amount of ADP produced from enzyme reaction without a separation step.
This kit demonstrates high sensitivity (<0.3 μM ADP) and broad ATP tolerance (1-300 μM. This kit can be utilized for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors.
This kit demonstrates high sensitivity (<0.3 μM ADP) and broad ATP tolerance (1-300 μM. This kit can be utilized for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
