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I4777

Sigma-Aldrich

Interferon-γ from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinonimo/i:

IFN-γ

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About This Item

Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

mouse

Livello qualitativo

Ricombinante

expressed in E. coli

Saggio

≥98% (SDS-PAGE)

Forma fisica

lyophilized powder

PM

protein 15.6 kDa

Confezionamento

pkg of 0.1 mg

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Colore

white

Solubilità

water: soluble 1.000 mL, clear, colorless

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

mouse ... Ifng(15978)

Applicazioni

Interferon-γ from mouse has been used:
  • for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c+ (CD11 antigen-like family member C) lung macrophages
  • for stimulation of murine macrophages to induce the production of nitrite and nitrate
  • to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation

Azioni biochim/fisiol

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.

Stato fisico

Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.

Risultati analitici

The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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S N Vogel et al.
Infection and immunity, 38(2), 681-685 (1982-11-01)
Silica has been used for many years as an agent which selectively alters macrophage functions and, as such, has been used to assess the role of macrophages in the immune response to a variety of microbial and chemically defined agents.
Sergio Arias-Negrete et al.
Analytical biochemistry, 328(1), 14-21 (2004-04-15)
Preparation of a nitrate reductase lysate of Escherichia coli MC1061 to measure nitrate and nitrite in biologic fluids is described. To obtain the crude bacterial lysate containing nitrate reductase activity, E. coli MC1061 was subjected to 16-20 freeze-thawing cycles, from
P W Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(19), 5842-5846 (1983-10-01)
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of
Anne M Sanders et al.
BMC cancer, 10, 121-121 (2010-04-02)
Spontaneous Regression/Complete Resistant (SR/CR) mice are a colony of cancer-resistant mice that can detect and rapidly destroy malignant cells with innate cellular immunity, predominately mediated by granulocytes. Our previous studies suggest that several effector mechanisms, such as perforin, granzymes, or
Marco Straccia et al.
Glia, 61(10), 1607-1619 (2013-07-31)
The eicosanoid prostaglandin E2 (PGE2 ) plays important roles in neuroinflammation and it is produced by the sequential action of the enzymes cyclooxygenase-2 (COX-2) and prostaglandin E synthase (PTGES). The expression of both enzymes and the production of PGE2 are

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