H8280
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow
matrix fast-flow highly cross-linked 4% beaded agarose
Sinonimo/i:
N-Hydroxysuccinimidyl-Agarose
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
5 ML
130,00 €
15 ML
283,00 €
130,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Scegli un formato
Cambia visualizzazione
5 ML
130,00 €
15 ML
283,00 €
About This Item
130,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Stato
isopropanol suspension
Grado di funzionalizzazione
16-23 μmol per mL
tecniche
protein array: suitable
Matrice
fast-flow highly cross-linked 4% beaded agarose
Gruppo funzionale matrice
N-hydroxysuccinimide active ester
Braccio spaziatore
6 atoms (when ligands are coupled by displacement of NHS)
Temperatura di conservazione
2-8°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.
Applicazioni
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.
Caratteristiche e vantaggi
- Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation, which gives a high degree of substitution of the selected ligand
- NHS coupling method enables chemically stable ligand attachment
Note legali
Sepharose is a trademark of Cytiva
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
3 - Flammable liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
53.6 °F
Punto d’infiammabilità (°C)
12 °C
Dispositivi di protezione individuale
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
