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GE27-0846-01

Thrombin Protease

Cytiva 27-0846-01

Sinonimo/i:

Proteolytic Enzyme, Thrombin Activity, Thrombin Enzyme

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1 PKG
169,00 €

169,00 €

Spedizione prevista il26 marzo 2025Dettagli

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Cambia visualizzazione
1 PKG
169,00 €

About This Item

Codice UNSPSC:
12352204
NACRES:
NA.56

169,00 €

Spedizione prevista il26 marzo 2025Dettagli

Confezionamento

pkg of 500 units

Produttore/marchio commerciale

Cytiva 27-0846-01

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3). Purified from bovine plasma.

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors (see pGEX Vectors (GST Gene Fusion System)) containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

Applicazioni

Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Caratteristiche e vantaggi

  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose™ High Performance, or Glutathione Sepharose™ 4 Fast Flow

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number

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Thrombin, Bovine

Sigma-Aldrich

605157

Thrombin, Bovine

Trombina lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

Sigma-Aldrich

T6884

Trombina

Proteasi TEV

Sigma-Aldrich

T4455

Proteasi TEV

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Articoli

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

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Protocolli

pGEX Vectors

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

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