Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

GE27-0843-01

PreScission Protease

Cytiva 27-0843-01

Sinonimo/i:

fusion protein, GST-tag removal

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

1 PKG
375,00 €

375,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
1 PKG
375,00 €

About This Item

Codice UNSPSC:
41106501
NACRES:
NA.56

375,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Confezionamento

pkg of 500 units

Produttore/marchio commerciale

Cytiva 27-0843-01

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Categorie correlate

Descrizione generale

PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step.

On-column or off-column enzyme cleavage

GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags. Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.

Vectors encoding the HRV 3C protease recognition sequence

pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.

Applicazioni

Specific, low temperature cleavage of fusion protein tags; Simple on-column cleavage during affinity purification
PreScission Protease has been used for eluting GST-tagged AT3, during in vitro ubiquitination reactions and AT3-Ub preparation.[1] It has also been used to cleave GST tag from the a-enolase during induction of GST a-enolase expression in the protease-deficient BL21 strain of Escherichia coli.[2]

Caratteristiche e vantaggi

  • Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
  • Time-saving – GST-tagged proteins can be cleaved while still bound to an affinity resin.
  • Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

pGEX Vectors
pGEX Vectors are to be used for scientific investigation and research and for no other purpose whatsoever and a license for commercial use of the licensed products and the processes claimed in US patent 5,654,176 and equivalent patents and patent applications in other countries must be negotiated directly with Millipore Corp (formerly Chemicon International Inc) by the purchaser prior to such use.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 8

1 of 8

SUMO Protease His tagged recombinant protein, lyophilized powder

Sigma-Aldrich

SAE0067

SUMO Protease

GST Spintrap™ Cytiva 28-9523-59

GE28-9523-59

GST Spintrap™

GSTrap™ Fast Flow Cytiva 17-5130-01, pack of 5 × 1 mL

GE17-5130-01

GSTrap™ Fast Flow

GST Bulk Kit Cytiva 27-4570-01, pack of 1 kit

GE27-4570-01

GST Bulk Kit

GSTrap™ Fast Flow Cytiva 17-5130-02, pack of 2 × 1 mL

GE17-5130-02

GSTrap™ Fast Flow

Glutathione Sepharose™ 4 Fast Flow Cytiva 17-5132-01, pack of 25 mL

GE17-5132-01

Glutathione Sepharose™ 4 Fast Flow

GST Buffer Kit Cytiva 28-9523-61, pack of 1 kit

GE28-9523-61

GST Buffer Kit

Glutathione Seph High Performance Cytiva 17-5279-02, pack of 100 mL

GE17-5279-02

Glutathione Seph High Performance

Zhen Wang et al.
Nature communications, 10(1), 3162-3162 (2019-07-20)
HECT E3 ligases control the degradation and functioning of numerous oncogenic/tumor-suppressive factors and signaling proteins, and their activities must be tightly regulated to prevent cancers and other diseases. Here we show that the Nedd4 family HECT E3 WWP1 adopts an
Dorothée Raoux-Barbot et al.
PloS one, 13(11), e0206133-e0206133 (2018-11-13)
Several bacterial pathogens produce nucleotidyl cyclase toxins to manipulate eukaryotic host cells. Inside host cells they are activated by endogenous cofactors to produce high levels of cyclic nucleotides (cNMPs). The ExoY toxin from Pseudomonas aeruginosa (PaExoY) and the ExoY-like module
Lingdi Zhang et al.
Nucleic acids research, 49(5), 2721-2739 (2021-02-13)
We recently found that toxic PS-ASOs can cause P54nrb and PSF nucleolar mislocalization in an RNase H1-dependent manner. To better understand the underlying mechanisms of these observations, here we utilize different biochemical approaches to demonstrate that PS-ASO binding can alter
Ji-Young Lee et al.
Cell reports, 27(12), 3602-3617 (2019-06-20)
The hepatitis C virus (HCV) is a major cause of chronic liver disease, affecting around 71 million people worldwide. Viral RNA replication occurs in a membranous compartment composed of double-membrane vesicles (DMVs), whereas virus particles are thought to form by
Ana Belén Uceda et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-03-07)
Frataxin is a mitochondrial protein which deficiency causes Friedreich's ataxia, a cardio-neurodegenerative disease. The lack of frataxin induces the dysregulation of mitochondrial iron homeostasis and oxidative stress, which finally causes the neuronal death. The mechanism through which frataxin regulates the
Visualizza tutti i documenti correlati

Articoli

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Vedi tutto

Protocolli

pGEX Vectors

This page describes the characteristics of pGEX expression vectors used with Cytiva products.

HiTrap® Benzamidine FF for Thrombin & Factor Xa Removal

Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.