GE17-0969-01
IgG Sepharose™ 6 Fast Flow
Cytiva 17-0969-01, pack of 10 mL
Sinonimo/i:
IgG Column
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647,00 €
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About This Item
647,00 €
Prodotti consigliati
ligand
human IgG
Confezionamento
pack of 10 mL
Produttore/marchio commerciale
Cytiva 17-0969-01
Condizioni di stoccaggio
(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)
Matrice
highly cross-linked 6% agarose
Diametro medio
90 μm
cleaning in place
3-12
Intervallo di esercizio
3-10
Capacità
~3 mg binding capacity(Protein A)
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.
Caratteristiche e vantaggi
- IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
- Binds at least 2 mg protein A/mL medium
- Suitable for tandem affinity purification (TAP) of protein complexes
Stoccaggio e stabilità
Risultati analitici
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
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Articoli
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocolli
This page shows how to purify protein A-tagged proteins using Cytiva products.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
Contenuto correlato
Per studiare in vitro le interazioni proteina-proteina potete ricorrere a saggi di pull-down per affinità, o GST pull-down, purificazione per affinità in tandem (TAP) e co-immunoprecipitazione.
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
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