G9145

[Leu¹⁵]-Gastrin I human

≥95% (HPLC)

• Sinonimo/i: [Leu¹⁵]-HG-17, LHG-17, [15-Leucine]-Gastrin I, human heptadecapeptide, [18-Pyroglutamic acid, 32-leucine]-Big gastrin I-(18-34)-peptide amide, human, [Leu¹⁵]-Gastrin I, human, [Leucine¹⁵]-Gastrin I heptadecapeptide, [Leucine¹⁵]-Little gastrin I
• Formula empirica (notazione di Hill): C₉₈H₁₂₆N₂₀O₃₁
• Numero CAS: 39024-57-2
• Peso molecolare: 2080.16
• Beilstein: 5227423
• Numero CE: 254-250-2
• Numero MDL: MFCD00076502
• Codice UNSPSC: 12352209
• ID PubChem: 24895364
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Saggio: ≥95% (HPLC)
• Stato: powder
• tecniche: cell culture | mammalian: suitable
• N° accesso UniProt: P01350
• Temperatura di conservazione: −20°C
• Stringa SMILE: CC(C)CC(NC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)C3CCCN3C(=O)CNC(=O)C4CCC(=O)N4)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(CCC(O)=O)C(=O)NC(C)C(=O)NC(Cc5ccc(O)cc5)C(=O)NCC(=O)NC(Cc6c[nH]c7ccccc67)C(=O)NC(CC(C)C)C(=O)NC(CC(O)=O)C(=O)NC(Cc8ccccc8)C(N)=O
• InChI: 1S/C98H126N20O31/c1-49(2)38-68(115-96(147)72(43-55-46-101-60-19-12-10-17-58(55)60)117-98(149)74-20-13-37-118(74)77(122)48-103-86(137)61-25-31-75(120)105-61)93(144)111-66(30-36-82(131)132)92(143)110-65(29-35-81(129)130)91(142)109-64(28-34-80(127)128)90(141)108-63(27-33-79(125)126)89(140)107-62(26-32-78(123)124)88(139)104-51(5)85(136)113-70(41-53-21-23-56(119)24-22-53)87(138)102-47-76(121)106-71(42-54-45-100-59-18-11-9-16-57(54)59)95(146)114-69(39-50(3)4)94(145)116-73(44-83(133)134)97(148)112-67(84(99)135)40-52-14-7-6-8-15-52/h6-12,14-19,21-24,45-46,49-51,61-74,100-101,119H,13,20,25-44,47-48H2,1-5H3,(H2,99,135)(H,102,138)(H,103,137)(H,104,139)(H,105,120)(H,106,121)(H,107,140)(H,108,141)(H,109,142)(H,110,143)(H,111,144)(H,112,148)(H,113,136)(H,114,146)(H,115,147)(H,116,145)(H,117,149)(H,123,124)(H,125,126)(H,127,128)(H,129,130)(H,131,132)(H,133,134); CMVMLPDUAGUTOC-UHFFFAOYSA-N
• Informazioni sul gene: human ... GAST(2520)

Descrizione

Amino Acid Sequence

Glp-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp-Leu-Asp-Phe-NH2

Descrizione generale

GAST gene encodes for the protein [Leu15]-Gastrin I in human. In human chromosome, the gene GAST is localized on 17q21.2.[1] G cells in the stomach antrum produces the precursor of [Leu15]-Gastrin I, progastrin.[1] Progastrin undergoes cleavage and processing to yield gastrin, which is trophic for the entire gastrointestinal epithelium. Gastrin is essential for the growth of the digestive system and stimulates the production of gastric acid by parietal cells. Gastrin exerts its function through G-protein-coupled receptor called the cholecystokinin (CCK) or CCK-B receptor (CCK-BR).[2] Gastrin release is stimulated by food, especially protein diet and is inhibited by very low pH. Atrophic gastritis, a Helicobacter pylori infection and long-term administration of proton pump inhibitors infection may cause overexpression of gastrin. Gastric adenocarcinoma show high levels of gastrin.[1]

Applicazioni

Gastrin has been used:

• as a medium supplement establish enteroid cultures[3]
• as a supplement to standard Dulbecco′s Modified Eagle Medium (DMEM) culture medium,[4][5] human minigut medium[6] to establish the growth of crypts.

Azioni biochim/fisiol

Gastrin I analog with full biological activity and improved stability in aqueous solutions. Leu¹⁵ replaces Met¹⁵, that is readily oxidized resulting in loss of biological activity.

Altre note

Lyophilized from 0.1% TFA in H2O

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)