EPI010
Sirtuin 2 (SIRT2) Inhibitor Screening Assay Kit
100 assays in 96 well plates
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.41
Prodotti consigliati
impiego
100 assays in 96 well plates
Condizioni di stoccaggio
protect from light
N° accesso NCBI
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... SIRT2(22933)
mouse ... SIRT2(64383)
Descrizione generale
Sirtuin 2 (SIRT2) is a cytoplasmic protein and is part of the sirtuin family of proteins.[1] Sirtuin family contains a sirtuin core domain and isgrouped into four classes with SIRT2 being a member of class I. SIRT2 colocalizes with microtubules.[2][3] The gene encoding this protein is localized on human chromosome 19q13.2.[4]
With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.
With Sigma′s Sirtuin Inhibitor Screening Kit, Sirtuin deacetylates the Substrate, and then a Developer cleaves the deacetylated substrate to release a fluorescent group, the latter of which can be detected at Ex/Em = 395/541 nm. In the presence of a SIRT inhibitor, the deacetylation will be impeded, preventing substrate cleavage and release of the fluorescent group. The kit provides a rapid, simple, sensitive, and reliable test, which is suitable for both low and high-throughput screening of SIRT2 inhibitors. A positive control inhibitor, Nicotinamide, is included to compare with the efficacy of the test inhibitors.
Applicazioni
Sirtuin 2 (SIRT2) inhibitor screening assay kit has been used for acetyl peptide deacetylase assay.[5]
Azioni biochim/fisiol
Sirtuin 2 (SIRT2) maintains the integrity of the genome.[1] Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington′s disease. Huntington′s disease is characterized by increased sterol synthesis in neuronal cells and this process is reversed by SIRT2 inhibition.[6] SIRT2 can deacetylate lys40 of α-tubulin both in vitro and in vivo. Knockdown of SIRT2 by small interfering RNA (siRNA) leads to tubulin hyperacetylation.[3]
Caratteristiche e vantaggi
- Simple, sensitive, and reliable assay
- Species reactivity: mammalian
- Utilizes fluorometric methods
- Suitable for individual tests or high throughput assays
- Convenient 96-well microplate format
- Screening and characterization of Sirtuin inhibitors
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
10 - Combustible liquids
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Proteomics-based identification of differentially expressed genes in human gliomas: down-regulation of SIRT2 gene
Masaharu Hiratsuka
Biochemical and Biophysical Research Communications (2003)
Damir Janigro
The Cell Cycle in the Central Nervous System (2008)
Propofol Inhibits SIRT2 Deacetylase through a Conformation-specific, Allosteric Site*
Brian P
The Journal of Biological Chemistry (2015)
The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase.
North BJ
Molecular and Cellular Biochemistry, 11(2) (2003)
Brian P Weiser et al.
The Journal of biological chemistry, 290(13), 8559-8568 (2015-02-11)
meta-Azi-propofol (AziPm) is a photoactive analog of the general anesthetic propofol. We photolabeled a myelin-enriched fraction from rat brain with [(3)H]AziPm and identified the sirtuin deacetylase SIRT2 as a target of the anesthetic. AziPm photolabeled three SIRT2 residues (Tyr(139), Phe(190)
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