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E4913

Sigma-Aldrich

Plant/Fungi RNA Purification Kit

greener alternative

sufficient for 50 purifications

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

impiego

sufficient for 50 purifications

Caratteristiche più verdi

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

, Aligned

Descrizione generale

Norgen Biotek′s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including viral RNA, from a wide range of plant and filamentous fungal species. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The procedure is rapid and convenient, as it does not rely on the use of liquid nitrogen in order to homogenize the samples.

Applicazioni

For the rapid purification of total RNA (including microRNA) from plants and fungi.
Purified RNA is of the highest quality and can be used in a number of downstream applications, including:
  • qRT-PCR
  • Reverse transcription PCR
  • Northern blotting
  • RNAse protection
  • Primer extension
  • Expression array assays
  • Next Generation Sequencing

Caratteristiche e vantaggi

  • Isolate RNA from a wide range of plant and filamentous fungi samples.
  • Robust lysis buffer is suitable for challenging samples such as pine needle or grape leaf.
  • Isolate a diversity of RNA species from large mRNA, microRNA, and viral RNA.
  • Purification of high yield and quality RNA.
  • No phenol/chloroform extraction required.
  • Liquid nitrogen is not required for homogenization

Altre note

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Exclamation markEnvironment
GHS07,GHS09

Avvertenze

Warning

Indicazioni di pericolo

H302 + H332,H315,H319,H410

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

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I El-Sharkawy et al.
Journal of experimental botany, 60(3), 907-922 (2009-02-14)
Seven ERF cDNAs were cloned from two Japanese plum (Prunus salicina L.) cultivars, 'Early Golden' (EG) and 'Shiro' (SH). Based on the sequence characterization, these Ps-ERFs could be classified into three of the four known ERF families. Their predicted amino
I El-Sharkawy et al.
Journal of experimental botany, 63(3), 1225-1239 (2011-11-15)
Several dwarf plum genotypes (Prunus salicina L.), due to deficiency of unknown gibberellin (GA) signalling, were identified. A cDNA encoding GA 2-oxidase (PslGA2ox), the major gibberellin catabolic enzyme in plants, was cloned and used to screen the GA-deficient hybrids. This
I El-Sharkawy et al.
Journal of experimental botany, 61(6), 1761-1770 (2010-03-06)
Germin-like proteins (GLPs) have several proposed roles in plant development and defence. Two novel genes (Ps-GLP1 and 2) encoding germin-like protein were isolated from plum (Prunus salicina). Their regulation was studied throughout fruit development and during ripening of early and
Novel variants of grapevine leafroll-associated virus 4 and 7 detected from a grapevine showing leafroll symptoms.
Takao Ito et al.
Archives of virology, 158(1), 273-275 (2012-08-29)
Hila Yaffe et al.
BMC research notes, 5, 45-45 (2012-01-21)
Rapid RNA extraction is commonly performed with commercial kits, which are very expensive and can involve toxic reagents. Most of these kits can be used with healthy plant tissues, but do not produce consistently high-quality RNA from necrotic fungus-infected tissues
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Protocolli

Plant RNA/DNA Purification Kit

The Plant RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and DNA simultaneously from a single sample of plants.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

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