Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

DNASE10

Sigma-Aldrich

On-Column DNase I Digestion Set

sufficient for 10 preparations

Sinonimo/i:

Deoxyribonuclease I Set

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

1 SET
60,90 €

60,90 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
1 SET
60,90 €

About This Item

Codice UNSPSC:
12352204
NACRES:
NA.52

60,90 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Grado

Molecular Biology

Livello qualitativo

200

Stato

liquid

impiego

sufficient for 10 preparations

tecniche

RNA purification: suitable

Temperatura di conservazione

−20°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit and the Spectrum Plant Total RNA Kit. It can also be used with many other commercially available RNA purification kits.
The vast majority of DNA is eliminated from total RNA purifications by the procedures provided in the standard protocols of the Sigma Kits named below. However, for very sensitive applications, such as quantitative RT-PCR, even trace amounts of DNA can result in false positive detection of cDNA-derived amplicons.

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

The term ′On-Column′ indicates that digestion is accomplished in-process for true convenience. Each of Sigma′s total RNA purification kits utilize a binding column for purification. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow. To review the complete protocol, see the Technical Bulletin for any of the Kits named above.

Applicazioni

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.[1]

Caratteristiche e vantaggi

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

Principio

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.

Note legali

GenElute is a trademark of Sigma-Aldrich Co. LLC
Spectrum is a trademark of Sigma-Aldrich Co. LLC

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

RTN350

Kit di minipreparazione dell′RNA totale di mammifero GenElute™, sufficient for 350 purifications

RTN70

Kit di minipreparazione dell′RNA totale di mammifero GenElute™, sufficient for 70 purifications

RTN10

Kit di minipreparazione dell′RNA totale di mammifero GenElute™, sufficient for 10 purifications

DNASE70

On-Column DNase I Digestion Set, sufficient for 70 preparations

STRN10

Kit per RNA totale vegetale Spectrum™, sufficient for 10 purifications

STRN50

Kit per RNA totale vegetale Spectrum™, sufficient for 50 purifications

STRN250

Kit per RNA totale vegetale Spectrum™, sufficient for 250 purifications

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLCR5423
SLCR1901
SLCN1907
SLCL4132
SLCF1818

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 3

1 of 3

DNasi I grade II, from bovine pancreas

Roche

10104159001

DNasi I

DNasi I from bovine pancreas

Roche

11284932001

DNasi I

OptiPrep™ mezzo a gradiente di densità used for cell and subcellular organelle isolation

Sigma-Aldrich

D1556

OptiPrep™ mezzo a gradiente di densità

Filipa Monteiro et al.
PloS one, 8(9), e72998-e72998 (2013-09-12)
The pivotal role of cultivated grapevine (Vitis vinifera L.) in many countries economy is compromised by its high susceptibility to Plasmopara viticola, the causal agent of downy mildew disease. Recent research has identified a set of genes related to resistance
Carmen Michán et al.
FEMS yeast research, 9(7), 1078-1090 (2009-08-26)
We report the absolute transcription profiles of 24 genes coding for putative thioredoxin (Trx)- and glutathione (GSH)-dependent redox system components, accompanying the Candida albicans batch culture growth, under either yeast or hyphal conditions. All mRNAs investigated (plus the housekeeping ACT1)
Michal Bukowski et al.
Nature communications, 4, 2012-2012 (2013-06-19)
Toxin-antitoxin systems were shown to be involved in plasmid maintenance when they were initially discovered, but other roles have been demonstrated since. Here we identify and characterize a novel toxin-antitoxin system (pemIKSa) located on Staphylococcus aureus plasmid pCH91. The toxin

Protocolli

Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits

Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.

Contenuto correlato

Spectrum™ Plant Total RNA Kit

The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.

Kit GenElute™-E per la purificazione di RNA e DNA

La tecnologia GenElute™-E Single Spin per la purificazione di DNA e RNA consente di estrarre e purificare rapidamente DNA e RNA da campioni di cellule mammifere, sangue e tessuti animali e vegetali.

GenElute™-E Kits for RNA and DNA Purification

GenElute™-E Single Spin DNA and RNA purification technology enables rapid DNA and RNA extraction and purification from mammalian cells, blood, tissue, and plant tissue samples.

Domande

Recensioni

Nessuna valutazione

Filtri attivi

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.