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E0887

Sigma-Aldrich

Esterase from rabbit liver

lyophilized powder, ≥30 units/mg protein

Sinonimo/i:

Carboxyl esterase, Carboxylic-ester hydrolase

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500 UNITS
222,00 €
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367,00 €

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500 UNITS
222,00 €
1000 UNITS
367,00 €

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

222,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Stato

lyophilized powder

Attività specifica

≥30 units/mg protein

PM

60 kDa

Composizione

Protein, ≥85% biuret

Temperatura di conservazione

−20°C

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Descrizione generale

Esterase belongs to the C-family of carboxylesterases.[1] It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.[2]

Applicazioni

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).
Esterase from rabbit liver has been used:
  • in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors[1]
  • in the catalysis and kinetic profiling studies of peptidic compounds[3]
  • in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)[4]
  • in the hydrolysis of oleuropein aglycone (Ole-AG)[5]

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.[6]

Azioni biochim/fisiol

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.[7]
Esterase enzyme has broad substrate specificity.[3] Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides.[8] Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.[9]

Definizione di unità

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Stato fisico

Lyophilized powder containing Tris buffer salts

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Hansjörg Schwertz et al.
Journal of cardiovascular pharmacology, 52(2), 151-160 (2008-08-02)
Animal data strongly support a role for inflammation in myocardial ischemia reperfusion injury. Attempts at cardioprotection by immunomodulation (such as with the specific C5 antibody pexelizumab) in humans have been disappointing. We hypothesized that a broader spectrum antiinflammatory agent might
Z Qiao et al.
The Journal of international medical research, 37(4), 1029-1037 (2009-09-19)
Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) contributes to atherosclerotic plaque instability and subsequent sudden coronary death. Statins are associated with decreased stroke risk and may improve stability of atherosclerotic plaques. The present study investigated the effect of simvastatin on expression of Lp-PLA(2)
Anna Sobeková et al.
Acta biologica Hungarica, 60(1), 45-54 (2009-04-22)
The activities of the antioxidant and detoxifying enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), glutathione reductase (GR), glutathione-S-transferase (GST), and the content of thiobarbituric acid reactive substances (TBARS) were determined in the liver and kidney of rabbits after
Sisi Zhang et al.
Current protocols, 2(7), e489-e489 (2022-07-21)
Polysorbates (PSs), including PS20 and PS80, are non-ionic surfactants widely used in the pharmaceutical industry to enhance drug solubility and stability. Despite their wide application, PSs are prone to degradation by either hydrolysis or oxidation in drug formulations during storage;
Shijin Zhang et al.
Langmuir : the ACS journal of surfaces and colloids, 36(22), 6261-6267 (2020-05-19)
To explore the potential of step-by-step assembly in the fabrication of biological materials, we designed and synthesized two peptide-based molecules for enzyme-instructed hierarchical assembly. Upon the treatment of alkaline phosphatase, one molecule undergoes enzyme-instructed self-assembly forming uniformed nanofibers. The other

Protocolli

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Domande

  1. Is it possible for the esterase from rabbit liver (E0887) to hydrolyze ethyl hexanoate into hexanoic acid?

    1 risposta
    1. The product has been evaluated for the conversion of ethyl butyrate to butyric acid and ethanol. However, there has been no evaluation for the hydrolysis of ethyl hexanoate into hexanoic acid, and there are no known citations of this product for ethyl hexanoate hydrolysis. Due to the structural similarity between ethyl hexanoate and ethyl butyrate, both containing an ester bond, it's possible that this product could hydrolyze ethyl hexanoate, as esterases typically accommodate a wide range of ester substrates. However, the rate and efficiency of the hydrolysis may differ from the enzyme activity tested using ethyl butyrate as a substrate.

      Utile?

Recensioni

Nessuna valutazione

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