D1054

Dihydrorhodamine 123

≥95% purity, powder

• Sinonimo/i: DHR 123; Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate; Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester
• Formula empirica (notazione di Hill): C₂₁H₁₈N₂O₃
• Numero CAS: 109244-58-8
• Peso molecolare: 346.38
• Beilstein: 5989965
• Numero MDL: MFCD00285712
• Codice UNSPSC: 12171500
• ID PubChem: 24893337
• NACRES: NA.47

Proprietà

• product name: Dihydrorhodamine 123, ≥95%
• Livello qualitativo: 200
• Saggio: ≥95%
• Forma fisica: powder
• Colore: pink
• Solubilità: DMSO: >5 mg/mL
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: −20°C
• Stringa SMILE: COC(=O)c1ccccc1C2c3ccc(N)cc3Oc4cc(N)ccc24
• InChI: 1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3; FNEZBBILNYNQGC-UHFFFAOYSA-N

Descrizione

Descrizione generale

Dihydrorhodamine 123 (DHR123) is an uncharged non-fluorescent dye and a derivative of rhodamine 123 (R123). Dihydrorhodamine 123 (DHR123) is oxidized to fluorescent R123 within cells in the presence of reactive oxygen species and it localizes in mitochondria. It is an analog of 2,7-Dichlorodihydrofluorescein (DCDHF), lipophilic and diffuses through cell membrane.

Applicazioni

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

Azioni biochim/fisiol

Dihydrorhodamine 123 detects superoxide ions at the single-cell level and is used for probing neutrophils superoxide generation in a respiratory burst event. It is also used for measuring oxidative stress in macrophages and human spermatozoa.

Dihydrorhodamine 123 is used for the detection of ROS (reactive oxygen species). Presence of ROS oxidizes dihydrorhodamine 123 to the fluorescent derivative rhodamine 123. The reaction happens in the presence of a peroxidase or a similar catalyst.

Substrati

Peroxidase substrate

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)