This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Applicazioni
Il terreno Eagle modificato di Dulbecco (DMEM) è una versione modificata del terreno basale Eagle (BME) che contiene concentrazioni quattro volte superiori di amminoacidi e vitamine. La formulazione originale conteneva 1000 mg/L di glucosio ed è stata utilizzata per coltivare cellule embrionali di topo. Da allora, il terreno è stato modificato in diversi modi per favorire le colture primarie di cellule di topo e pollo e di molte altre cellule normali e trasformate. Ognuno di questi terreni offre una diversa combinazione di L-glutammina e piruvato di sodio. Inoltre, i livelli di glucosio sono stati innalzati a 4500 mg/L, motivo per cui il terreno è denominato “DMEM/High”.
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American journal of physiology. Heart and circulatory physiology, 320(3), H1156-H1169 (2021-01-16)
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