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CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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About This Item

Numero CE:
217-896-6
Codice UNSPSC:
12352207
NACRES:
NA.32

Livello qualitativo

200

impiego

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

tecniche

flow cytometry: suitable
protein staining: suitable

Fluorescenza

λex 490 nm; λem 590 nm (JC-1 dye)

applicazioni

cell analysis
detection

Metodo di rivelazione

fluorometric

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Categorie correlate

Applicazioni

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Caratteristiche e vantaggi

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principio

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Risultati analitici

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Solo come componenti del kit

N° Catalogo
Descrizione
  • DMSO 1 mL
  • JC-1 Assay Buffer 5X 25 mL
  • JC-1 Stain 25 μg
  • Valinomycin Ready Made 100 μL

Pittogrammi

Skull and crossbones
GHS06

Avvertenze

Danger

Indicazioni di pericolo

H300 + H310

Classi di pericolo

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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