C9521

Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride

kallikrein substrate, ≥95% (HPLC), powder

• Sinonimo/i: Z-Phe-Arg-AMC
• Formula empirica (notazione di Hill): C₃₃H₃₆N₆O₆ · HCl
• Numero CAS: 70382-26-2
• Peso molecolare: 649.14
• Numero MDL: MFCD00077030
• Codice UNSPSC: 12352204
• ID PubChem: 24893165
• NACRES: NA.32

Proprietà

• Nome del prodotto: Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride, kallikrein substrate
• Saggio: ≥95% (HPLC)
• Stato: powder
• Concentrazione: ≥95%
• Solubilità: methanol: 20 mg/mL, clear, colorless
• Temperatura di conservazione: −20°C
• Stringa SMILE: O=C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](CCCNC(N)=N)C(NC2=CC=C(C(C)=CC(O3)=O)C3=C2)=O)=O)OCC4=CC=CC=C4.[Cl]
• InChI: 1S/C33H36N6O6/c1-21-17-29(40)45-28-19-24(14-15-25(21)28)37-30(41)26(13-8-16-36-32(34)35)38-31(42)27(18-22-9-4-2-5-10-22)39-33(43)44-20-23-11-6-3-7-12-23/h2-7,9-12,14-15,17,19,26-27H,8,13,16,18,20H2,1H3,(H,37,41)(H,38,42)(H,39,43)(H4,34,35,36); ZZGDDBWFXDMARY-UHFFFAOYSA-N

Descrizione

Descrizione generale

Z-Phe-Arg 7-amido-4-methylcoumarin (Z-FR-AMC) is a peptidomimetic substrate for papain[1]and other enzymes such as cathepsin K.[2] It is also a fluorogenic synthetic peptide for the enzymes cathepsins L and B.[3]

Applicazioni

Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride has been used:

• as a fluorogenic substrate in actinidin inhibition assay[4]
• as a kallikrein substrate[5]
• as a trypsin substrate for fluorometric assay[6]
• as a cathepsin-L substrate[7]

Azioni biochim/fisiol

Z-Phe-Arg 7-amido-4-methylcoumarin (Z-FR-AMC) proteolytic lysis by proteases leads to the liberation of AMC resulting in increased fluorescence in the enzymatic reaction.[1]

Substrati

A fluorogenic substrate for plasma kallikrein.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)